Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation
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Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. / Dahl, Marius A; Areta, José Lisandro; Jeppesen, Per Bendix; Birk, Jesper Bratz; Johansen, Egil I; Ingemann-Hansen, Thorsten; Hansen, Mette; Skålhegg, Bjørn Steen; Ivy, John L; Wojtaszewski, Jørgen; Overgaard, Kristian; Jensen, Jørgen.
I: Journal of Applied Physiology, Bind 129, Nr. 2, 2020, s. 297-310.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation
AU - Dahl, Marius A
AU - Areta, José Lisandro
AU - Jeppesen, Per Bendix
AU - Birk, Jesper Bratz
AU - Johansen, Egil I
AU - Ingemann-Hansen, Thorsten
AU - Hansen, Mette
AU - Skålhegg, Bjørn Steen
AU - Ivy, John L
AU - Wojtaszewski, Jørgen
AU - Overgaard, Kristian
AU - Jensen, Jørgen
N1 - CURIS 2020 NEXS 258
PY - 2020
Y1 - 2020
N2 - The present study compared the effects of post exercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO) only supplementation on muscle glycogen metabolism, anabolic cell signalling and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol·kg dw-1) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after despite substantial glycogen synthesis (176.1±19.1 and 204.6±27.0 mmol·kg dw-1 in CHO and CHO+PROT, respectively; p=0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5 h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared to CHO supplementation (54.6±11.0 vs 46.1±9.8 min; p=0.009). After the performance test, muscle glycogen was equally reduced in PRO+CHO and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389 or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6±7.6 mg nitrogen·kg-1, p=0.04) and higher than CHO (-10.7±6.3 mg nitrogen·kg-1, p=0.009). Conclusion: CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.
AB - The present study compared the effects of post exercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO) only supplementation on muscle glycogen metabolism, anabolic cell signalling and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol·kg dw-1) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after despite substantial glycogen synthesis (176.1±19.1 and 204.6±27.0 mmol·kg dw-1 in CHO and CHO+PROT, respectively; p=0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5 h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared to CHO supplementation (54.6±11.0 vs 46.1±9.8 min; p=0.009). After the performance test, muscle glycogen was equally reduced in PRO+CHO and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389 or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6±7.6 mg nitrogen·kg-1, p=0.04) and higher than CHO (-10.7±6.3 mg nitrogen·kg-1, p=0.009). Conclusion: CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.
KW - Faculty of Science
KW - Exercise
KW - Glycogen synthase
KW - Akt/PKB
KW - Protein synthesis
KW - Nitrogen balance
U2 - 10.1152/japplphysiol.00817.2019
DO - 10.1152/japplphysiol.00817.2019
M3 - Journal article
C2 - 32584664
VL - 129
SP - 297
EP - 310
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 2
ER -
ID: 243852329