TY - JOUR

T1 - Click chemistry mediated functionalization of vertical nanowires for biological applications

AU - Vutti, Surendra

AU - Schoffelen, Sanne

AU - Bolinsson, Jessica

AU - Buch-Månson, Nina

AU - Bovet, Nicolas Emile

AU - Nygård, Jesper

AU - Martinez, Karen Laurence

AU - Meldal, Morten Peter

N1 - [QDev]

PY - 2016

Y1 - 2016

N2 - Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the CuI-catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.

AB - Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the CuI-catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.

KW - alkyne-azide cycloaddition

KW - biosensors

KW - diazotransfer

KW - nanowires

KW - protein immobilization

U2 - 10.1002/chem.201504540

DO - 10.1002/chem.201504540

M3 - Letter

C2 - 26601641

AN - SCOPUS:84953282873

VL - 22

SP - 496

EP - 500

JO - Chemistry: A European Journal

JF - Chemistry: A European Journal

SN - 0947-6539

IS - 2

ER -