Chemical Genetic Screen for AMPKα2 Substrates Uncovers a Network of Proteins Involved in Mitosis

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

  • Max R. Banko
  • Jasmina J. Allen
  • Bethany E. Schaffer
  • Erik W. Wilker
  • Peiling P. Tsou
  • Jamie L. White
  • Judit Villén
  • Beatrice Wang
  • Sara R. Kim
  • Sakamoto, Kei
  • Steven P. Gygi
  • Lewis C. Cantley
  • Michael B. Yaffe
  • Kevan M. Shokat
  • Anne Brunet

The energy-sensing AMP-activated protein kinase (AMPK) is activated by low nutrient levels. Functions of AMPK, other than its role in cellular metabolism, are just beginning to emerge. Here we use a chemical genetics screen to identify direct substrates of AMPK in human cells. We find that AMPK phosphorylates 28 previously unidentified substrates, several of which are involved in mitosis and cytokinesis. We identify the residues phosphorylated by AMPK in vivo in several substrates, including protein phosphatase 1 regulatory subunit 12C (PPP1R12C) and p21-activated protein kinase (PAK2). AMPK-induced phosphorylation is necessary for PPP1R12C interaction with 14-3-3 and phosphorylation of myosin regulatory light chain. Both AMPK activity and PPP1R12C phosphorylation are increased in mitotic cells and are important for mitosis completion. These findings suggest that AMPK coordinates nutrient status with mitosis completion, which may be critical for the organism's response to low nutrients during development, or in adult stem and cancer cells.

OriginalsprogEngelsk
TidsskriftMolecular Cell
Vol/bind44
Udgave nummer6
Sider (fra-til)878-892
Antal sider15
ISSN1097-2765
DOI
StatusUdgivet - 23 dec. 2011
Eksternt udgivetJa

ID: 239567781