Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis
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Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis. / Hammerum, A M; Fussing, V; Aarestrup, F M; Wegener, Henrik Caspar.
I: Journal of Antimicrobial Chemotherapy, Bind 45, Nr. 5, 05.2000, s. 677-80.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Characterization of vancomycin-resistant and vancomycin-susceptible Enterococcus faecium isolates from humans, chickens and pigs by RiboPrinting and pulsed-field gel electrophoresis
AU - Hammerum, A M
AU - Fussing, V
AU - Aarestrup, F M
AU - Wegener, Henrik Caspar
PY - 2000/5
Y1 - 2000/5
N2 - Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and SmaI pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.
AB - Forty-eight vancomycin-resistant and 35 vancomycin-sensitive Danish Enterococcus faecium isolates obtained from pigs, chickens and humans, as well as the human vanA reference isolate BM4147, were characterized by EcoRI RiboPrinting and SmaI pulsed-field gel electrophoresis. RiboPrinting of the 84 isolates yielded 40 types whereas PFGE-typing yielded 57 types discriminated by differences in more than three bands. By molecular typing, both clonal spread of E. faecium as well as horizontal transmission of Tn1546 between animals and humans was supported. Furthermore, it was found that the population of E. faecium spreads freely between the animal and human reservoir.
KW - Animals
KW - Anti-Bacterial Agents
KW - Bacterial Proteins
KW - Bacterial Typing Techniques
KW - Carbon-Oxygen Ligases
KW - Chickens
KW - Deoxyribonuclease EcoRI
KW - Deoxyribonucleases, Type II Site-Specific
KW - Electrophoresis, Gel, Pulsed-Field
KW - Enterococcus faecium
KW - Gram-Positive Bacterial Infections
KW - Humans
KW - Poultry Diseases
KW - Restriction Mapping
KW - Swine
KW - Swine Diseases
KW - Vancomycin
KW - Vancomycin Resistance
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 10797092
VL - 45
SP - 677
EP - 680
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 5
ER -
ID: 172849734