Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes

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Standard

Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes. / Ecklu-Mensah, Gertrude; Olsen, Rebecca W.; Bengtsson, Anja; Ofori, Michael F.; Hviid, Lars; Jensen, Anja T.R.; Adams, Yvonne.

I: PLoS ONE, Bind 13, Nr. 10, e0204177, 2018.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ecklu-Mensah, G, Olsen, RW, Bengtsson, A, Ofori, MF, Hviid, L, Jensen, ATR & Adams, Y 2018, 'Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes', PLoS ONE, bind 13, nr. 10, e0204177. https://doi.org/10.1371/journal.pone.0204177

APA

Ecklu-Mensah, G., Olsen, R. W., Bengtsson, A., Ofori, M. F., Hviid, L., Jensen, A. T. R., & Adams, Y. (2018). Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes. PLoS ONE, 13(10), [e0204177]. https://doi.org/10.1371/journal.pone.0204177

Vancouver

Ecklu-Mensah G, Olsen RW, Bengtsson A, Ofori MF, Hviid L, Jensen ATR o.a. Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes. PLoS ONE. 2018;13(10). e0204177. https://doi.org/10.1371/journal.pone.0204177

Author

Ecklu-Mensah, Gertrude ; Olsen, Rebecca W. ; Bengtsson, Anja ; Ofori, Michael F. ; Hviid, Lars ; Jensen, Anja T.R. ; Adams, Yvonne. / Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes. I: PLoS ONE. 2018 ; Bind 13, Nr. 10.

Bibtex

@article{5c9bcc6770e546afa398032bcb8f6ef9,
title = "Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes",
abstract = "The lack of suitable animal models for the study of cytoadhesion of P. falciparum-infected erythrocytes (IEs) has necessitated in vitro studies employing a range of cell lines of either human tumour origin (e.g., BeWo and C32 cells) or non-human origin (e.g., CHO cells). Of the human cells available, many were isolated from adults, or derived from a pool of donors (e.g., HBEC-5i). Here we demonstrate, for the first time, the successful isolation of blood outgrowth endothelial cells (BOECs) from frozen stabilates of peripheral blood mononuclear cells obtained from small-volume peripheral blood samples from paediatric malaria patients. BOECs are a sub-population of human endothelial cells, found within the peripheral blood. We demonstrate that these cells express receptors such as Intercellular Adhesion Molecule 1 (ICAM-1/CD54), Endothelial Protein C Receptor (EPCR/CD201), platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31), Thrombomodulin (CD141), and support adhesion of P. falciparum IEs.",
author = "Gertrude Ecklu-Mensah and Olsen, {Rebecca W.} and Anja Bengtsson and Ofori, {Michael F.} and Lars Hviid and Jensen, {Anja T.R.} and Yvonne Adams",
year = "2018",
doi = "10.1371/journal.pone.0204177",
language = "English",
volume = "13",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

RIS

TY - JOUR

T1 - Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes

AU - Ecklu-Mensah, Gertrude

AU - Olsen, Rebecca W.

AU - Bengtsson, Anja

AU - Ofori, Michael F.

AU - Hviid, Lars

AU - Jensen, Anja T.R.

AU - Adams, Yvonne

PY - 2018

Y1 - 2018

N2 - The lack of suitable animal models for the study of cytoadhesion of P. falciparum-infected erythrocytes (IEs) has necessitated in vitro studies employing a range of cell lines of either human tumour origin (e.g., BeWo and C32 cells) or non-human origin (e.g., CHO cells). Of the human cells available, many were isolated from adults, or derived from a pool of donors (e.g., HBEC-5i). Here we demonstrate, for the first time, the successful isolation of blood outgrowth endothelial cells (BOECs) from frozen stabilates of peripheral blood mononuclear cells obtained from small-volume peripheral blood samples from paediatric malaria patients. BOECs are a sub-population of human endothelial cells, found within the peripheral blood. We demonstrate that these cells express receptors such as Intercellular Adhesion Molecule 1 (ICAM-1/CD54), Endothelial Protein C Receptor (EPCR/CD201), platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31), Thrombomodulin (CD141), and support adhesion of P. falciparum IEs.

AB - The lack of suitable animal models for the study of cytoadhesion of P. falciparum-infected erythrocytes (IEs) has necessitated in vitro studies employing a range of cell lines of either human tumour origin (e.g., BeWo and C32 cells) or non-human origin (e.g., CHO cells). Of the human cells available, many were isolated from adults, or derived from a pool of donors (e.g., HBEC-5i). Here we demonstrate, for the first time, the successful isolation of blood outgrowth endothelial cells (BOECs) from frozen stabilates of peripheral blood mononuclear cells obtained from small-volume peripheral blood samples from paediatric malaria patients. BOECs are a sub-population of human endothelial cells, found within the peripheral blood. We demonstrate that these cells express receptors such as Intercellular Adhesion Molecule 1 (ICAM-1/CD54), Endothelial Protein C Receptor (EPCR/CD201), platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31), Thrombomodulin (CD141), and support adhesion of P. falciparum IEs.

U2 - 10.1371/journal.pone.0204177

DO - 10.1371/journal.pone.0204177

M3 - Journal article

C2 - 30300360

AN - SCOPUS:85054779758

VL - 13

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 10

M1 - e0204177

ER -

ID: 208874102