Biomarkers of Skin Graft Healing in Venous Leg Ulcers
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There is a need for biomarkers that predict the success of transplantation of venous leg ulcers (with autologous split-thickness skin grafts). The primary objective of this exploratory study was to investigate the association between split-thickness skin graft healing in venous leg ulcers and candidate wound fluid biomarkers representing inflammatory cell and endogenous proteinase activities, and bioactivity. A secondary objective was to compare biomarker levels of the 17 venous leg ulcers with sterile split-thickness skin graft donor-site wounds in another 10 patients with venous leg ulcers. Wound fluids were collected for 24 h using a validated method. The concentration of preoperative matrix metalloproteinase-9 in wound fluid was higher in venous leg ulcers showing good healing (n = 10) than in venous leg ulcers showing poor healing (n = 7) 12 weeks after transplantation with meshed split-thickness skin grafts. The diagnostic value of matrix metalloproteinase-9 was good according to receiver-operating characteristic curve analysis. Matrix metalloproteinase activity in wound fluids from split-thickness skin graft donor-site wounds increased as a function of time and healing, but was still lower than matrix metalloproteinase activity in venous leg ulcer wound fluids, which showed increased levels of most biomarkers except for matrix metalloproteinase-9 and matrix metalloproteinase-2. In conclusion, wound fluid matrix metalloproteinase-9 concentration is a potential predictive biomarker of split-thickness skin graft healing in venous leg ulcers.
Originalsprog | Engelsk |
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Artikelnummer | adv00749 |
Tidsskrift | Acta Dermato-Venereologica |
Vol/bind | 102 |
Antal sider | 9 |
ISSN | 0001-5555 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
The authors thank the staff at the Copenhagen Wound Healing Center for their help in recruiting patients and collecting samples. This work has not been externally funded. Woundchek® was provided by Woundchek Laboratories. Akribes Biomedical GmbH performed the HNE, MPO and MMP assays, and the proliferation assays.
Publisher Copyright:
© 2022, Medical Journals/Acta D-V. All rights reserved.
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