Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling

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Standard

Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling. / Greenhalgh, Christopher J; Metcalf, Donald; Thaus, Anne L; Corbin, Jason E; Uren, Rachel; Morgan, Phillip O; Fabri, Louis J; Zhang, Jian-Guo; Martin, Helene M; Willson, Tracy A; Billestrup, Nils; Nicola, Nicos A; Baca, Manuel; Alexander, Warren S; Hilton, Douglas J.

I: The Journal of Biological Chemistry, Bind 277, Nr. 43, 25.10.2002, s. 40181-4.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Greenhalgh, CJ, Metcalf, D, Thaus, AL, Corbin, JE, Uren, R, Morgan, PO, Fabri, LJ, Zhang, J-G, Martin, HM, Willson, TA, Billestrup, N, Nicola, NA, Baca, M, Alexander, WS & Hilton, DJ 2002, 'Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling', The Journal of Biological Chemistry, bind 277, nr. 43, s. 40181-4. https://doi.org/10.1074/jbc.C200450200

APA

Greenhalgh, C. J., Metcalf, D., Thaus, A. L., Corbin, J. E., Uren, R., Morgan, P. O., Fabri, L. J., Zhang, J-G., Martin, H. M., Willson, T. A., Billestrup, N., Nicola, N. A., Baca, M., Alexander, W. S., & Hilton, D. J. (2002). Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling. The Journal of Biological Chemistry, 277(43), 40181-4. https://doi.org/10.1074/jbc.C200450200

Vancouver

Greenhalgh CJ, Metcalf D, Thaus AL, Corbin JE, Uren R, Morgan PO o.a. Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling. The Journal of Biological Chemistry. 2002 okt. 25;277(43):40181-4. https://doi.org/10.1074/jbc.C200450200

Author

Greenhalgh, Christopher J ; Metcalf, Donald ; Thaus, Anne L ; Corbin, Jason E ; Uren, Rachel ; Morgan, Phillip O ; Fabri, Louis J ; Zhang, Jian-Guo ; Martin, Helene M ; Willson, Tracy A ; Billestrup, Nils ; Nicola, Nicos A ; Baca, Manuel ; Alexander, Warren S ; Hilton, Douglas J. / Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling. I: The Journal of Biological Chemistry. 2002 ; Bind 277, Nr. 43. s. 40181-4.

Bibtex

@article{65e25ca1cb5b407693d6e035bd4dcf36,
title = "Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling",
abstract = "Suppressor of cytokine signaling (SOCS)-2 is a member of a family of intracellular proteins implicated in the negative regulation of cytokine signaling. The generation of SOCS-2-deficient mice, which grow to one and a half times the size of their wild-type littermates, suggests that SOCS-2 may attenuate growth hormone (GH) signaling. In vitro studies indicate that, while SOCS-2 can inhibit GH action at low concentrations, at higher concentrations it may potentiate signaling. To determine whether a similar enhancement of signaling is observed in vivo or alternatively whether increased SOCS-2 levels repress growth in vivo, we generated and analyzed transgenic mice that overexpress SOCS-2 from a human ubiquitin C promoter. These mice are not growth-deficient and are, in fact, significantly larger than wild-type mice. The overexpressed SOCS-2 was found to bind to endogenous GH receptors in a number of mouse organs, while phosphopeptide binding studies with recombinant SOCS-2 defined phosphorylated tyrosine 595 on the GH receptor as the site of interaction. Together, the data implicate SOCS-2 as having dual effects on GH signaling in vivo.",
keywords = "Animals, DNA-Binding Proteins, Growth Hormone, Mice, Mice, Transgenic, Protein Binding, Proteins, Receptors, Somatotropin, Recombinant Proteins, Repressor Proteins, Signal Transduction, Suppressor of Cytokine Signaling Proteins, Trans-Activators",
author = "Greenhalgh, {Christopher J} and Donald Metcalf and Thaus, {Anne L} and Corbin, {Jason E} and Rachel Uren and Morgan, {Phillip O} and Fabri, {Louis J} and Jian-Guo Zhang and Martin, {Helene M} and Willson, {Tracy A} and Nils Billestrup and Nicola, {Nicos A} and Manuel Baca and Alexander, {Warren S} and Hilton, {Douglas J}",
year = "2002",
month = oct,
day = "25",
doi = "10.1074/jbc.C200450200",
language = "English",
volume = "277",
pages = "40181--4",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "43",

}

RIS

TY - JOUR

T1 - Biological evidence that SOCS-2 can act either as an enhancer or suppressor of growth hormone signaling

AU - Greenhalgh, Christopher J

AU - Metcalf, Donald

AU - Thaus, Anne L

AU - Corbin, Jason E

AU - Uren, Rachel

AU - Morgan, Phillip O

AU - Fabri, Louis J

AU - Zhang, Jian-Guo

AU - Martin, Helene M

AU - Willson, Tracy A

AU - Billestrup, Nils

AU - Nicola, Nicos A

AU - Baca, Manuel

AU - Alexander, Warren S

AU - Hilton, Douglas J

PY - 2002/10/25

Y1 - 2002/10/25

N2 - Suppressor of cytokine signaling (SOCS)-2 is a member of a family of intracellular proteins implicated in the negative regulation of cytokine signaling. The generation of SOCS-2-deficient mice, which grow to one and a half times the size of their wild-type littermates, suggests that SOCS-2 may attenuate growth hormone (GH) signaling. In vitro studies indicate that, while SOCS-2 can inhibit GH action at low concentrations, at higher concentrations it may potentiate signaling. To determine whether a similar enhancement of signaling is observed in vivo or alternatively whether increased SOCS-2 levels repress growth in vivo, we generated and analyzed transgenic mice that overexpress SOCS-2 from a human ubiquitin C promoter. These mice are not growth-deficient and are, in fact, significantly larger than wild-type mice. The overexpressed SOCS-2 was found to bind to endogenous GH receptors in a number of mouse organs, while phosphopeptide binding studies with recombinant SOCS-2 defined phosphorylated tyrosine 595 on the GH receptor as the site of interaction. Together, the data implicate SOCS-2 as having dual effects on GH signaling in vivo.

AB - Suppressor of cytokine signaling (SOCS)-2 is a member of a family of intracellular proteins implicated in the negative regulation of cytokine signaling. The generation of SOCS-2-deficient mice, which grow to one and a half times the size of their wild-type littermates, suggests that SOCS-2 may attenuate growth hormone (GH) signaling. In vitro studies indicate that, while SOCS-2 can inhibit GH action at low concentrations, at higher concentrations it may potentiate signaling. To determine whether a similar enhancement of signaling is observed in vivo or alternatively whether increased SOCS-2 levels repress growth in vivo, we generated and analyzed transgenic mice that overexpress SOCS-2 from a human ubiquitin C promoter. These mice are not growth-deficient and are, in fact, significantly larger than wild-type mice. The overexpressed SOCS-2 was found to bind to endogenous GH receptors in a number of mouse organs, while phosphopeptide binding studies with recombinant SOCS-2 defined phosphorylated tyrosine 595 on the GH receptor as the site of interaction. Together, the data implicate SOCS-2 as having dual effects on GH signaling in vivo.

KW - Animals

KW - DNA-Binding Proteins

KW - Growth Hormone

KW - Mice

KW - Mice, Transgenic

KW - Protein Binding

KW - Proteins

KW - Receptors, Somatotropin

KW - Recombinant Proteins

KW - Repressor Proteins

KW - Signal Transduction

KW - Suppressor of Cytokine Signaling Proteins

KW - Trans-Activators

U2 - 10.1074/jbc.C200450200

DO - 10.1074/jbc.C200450200

M3 - Journal article

C2 - 12208853

VL - 277

SP - 40181

EP - 40184

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 43

ER -

ID: 132900007