Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2

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Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2. / Daugvilaite, Viktorija; Madsen, Christian Medom; Lückmann, Michael; Castello Echeverria, Clara; Sailer, Andreas Walter; Frimurer, Thomas Michael; Rosenkilde, Mette Marie; Benned-Jensen, Tau.

I: British Journal of Pharmacology, Bind 174, Nr. 13, 2017, s. 2031–2042.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Daugvilaite, V, Madsen, CM, Lückmann, M, Castello Echeverria, C, Sailer, AW, Frimurer, TM, Rosenkilde, MM & Benned-Jensen, T 2017, 'Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2', British Journal of Pharmacology, bind 174, nr. 13, s. 2031–2042. https://doi.org/10.1111/bph.13801

APA

Daugvilaite, V., Madsen, C. M., Lückmann, M., Castello Echeverria, C., Sailer, A. W., Frimurer, T. M., ... Benned-Jensen, T. (2017). Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2. British Journal of Pharmacology, 174(13), 2031–2042. https://doi.org/10.1111/bph.13801

Vancouver

Daugvilaite V, Madsen CM, Lückmann M, Castello Echeverria C, Sailer AW, Frimurer TM o.a. Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2. British Journal of Pharmacology. 2017;174(13):2031–2042. https://doi.org/10.1111/bph.13801

Author

Daugvilaite, Viktorija ; Madsen, Christian Medom ; Lückmann, Michael ; Castello Echeverria, Clara ; Sailer, Andreas Walter ; Frimurer, Thomas Michael ; Rosenkilde, Mette Marie ; Benned-Jensen, Tau. / Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2. I: British Journal of Pharmacology. 2017 ; Bind 174, Nr. 13. s. 2031–2042.

Bibtex

@article{a7b669a9e7e64e9a8e098cee639368fe,
title = "Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2",
abstract = "BACKGROUND AND PURPOSE: The G protein-coupled receptor Epstein Barr virus-induced gene 2 (EBI2, also known as GPR183) is activated by oxysterols and plays a pivotal role for proper B cell migration during immune responses. While the molecular basis of agonist binding has been addressed in several studies, the concept of biased agonism of EBI2 has not been explored.EXPERIMENTAL APPROACH: We investigated the effects of the EBI2 endogenous agonist 7α,25-OHC on G protein-dependent and -independent pathways as well as sodium ion allosterism using site-directed mutagenesis and functional studies. Moreover, we generated a homology model of EBI2 to investigate the structural basis of the allosteric modulation by sodium.KEY RESULTS: We show that residue N114, located in the middle of TM-III at position III:11/3.35, functions as an efficacy switch. Thus, substituting N114 with an alanine (N114A) completely abolishes Gαi activation by 7α,25-OHC even though the specific binding of the [(3) H]-7α,25-OHC radioligand increases. In contrast, the N114A mutant is still able to recruit β-arrestin and even with enhanced the potency (18.7-fold) compared to EBI2 WT. Underscoring the key role of N114, we also show that sodium has an negative allosteric effect on oxysterol binding and that this is mediated via N114. This is further supported by molecular modelling of the ion binding site based on a EBI2 homology model. Conclusions and Implications Collectively, our data points to N114 as a key residue for EBI2 signaling controlling the balance between G protein-dependent and -independent pathways and facilitating sodium binding.",
author = "Viktorija Daugvilaite and Madsen, {Christian Medom} and Michael L{\"u}ckmann and {Castello Echeverria}, Clara and Sailer, {Andreas Walter} and Frimurer, {Thomas Michael} and Rosenkilde, {Mette Marie} and Tau Benned-Jensen",
note = "This article is protected by copyright. All rights reserved.",
year = "2017",
doi = "10.1111/bph.13801",
language = "English",
volume = "174",
pages = "2031–2042",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
publisher = "Wiley",
number = "13",

}

RIS

TY - JOUR

T1 - Biased agonism and allosteric modulation of GPR183- a 7TM receptor also known as EBV-induced EBI2

AU - Daugvilaite, Viktorija

AU - Madsen, Christian Medom

AU - Lückmann, Michael

AU - Castello Echeverria, Clara

AU - Sailer, Andreas Walter

AU - Frimurer, Thomas Michael

AU - Rosenkilde, Mette Marie

AU - Benned-Jensen, Tau

N1 - This article is protected by copyright. All rights reserved.

PY - 2017

Y1 - 2017

N2 - BACKGROUND AND PURPOSE: The G protein-coupled receptor Epstein Barr virus-induced gene 2 (EBI2, also known as GPR183) is activated by oxysterols and plays a pivotal role for proper B cell migration during immune responses. While the molecular basis of agonist binding has been addressed in several studies, the concept of biased agonism of EBI2 has not been explored.EXPERIMENTAL APPROACH: We investigated the effects of the EBI2 endogenous agonist 7α,25-OHC on G protein-dependent and -independent pathways as well as sodium ion allosterism using site-directed mutagenesis and functional studies. Moreover, we generated a homology model of EBI2 to investigate the structural basis of the allosteric modulation by sodium.KEY RESULTS: We show that residue N114, located in the middle of TM-III at position III:11/3.35, functions as an efficacy switch. Thus, substituting N114 with an alanine (N114A) completely abolishes Gαi activation by 7α,25-OHC even though the specific binding of the [(3) H]-7α,25-OHC radioligand increases. In contrast, the N114A mutant is still able to recruit β-arrestin and even with enhanced the potency (18.7-fold) compared to EBI2 WT. Underscoring the key role of N114, we also show that sodium has an negative allosteric effect on oxysterol binding and that this is mediated via N114. This is further supported by molecular modelling of the ion binding site based on a EBI2 homology model. Conclusions and Implications Collectively, our data points to N114 as a key residue for EBI2 signaling controlling the balance between G protein-dependent and -independent pathways and facilitating sodium binding.

AB - BACKGROUND AND PURPOSE: The G protein-coupled receptor Epstein Barr virus-induced gene 2 (EBI2, also known as GPR183) is activated by oxysterols and plays a pivotal role for proper B cell migration during immune responses. While the molecular basis of agonist binding has been addressed in several studies, the concept of biased agonism of EBI2 has not been explored.EXPERIMENTAL APPROACH: We investigated the effects of the EBI2 endogenous agonist 7α,25-OHC on G protein-dependent and -independent pathways as well as sodium ion allosterism using site-directed mutagenesis and functional studies. Moreover, we generated a homology model of EBI2 to investigate the structural basis of the allosteric modulation by sodium.KEY RESULTS: We show that residue N114, located in the middle of TM-III at position III:11/3.35, functions as an efficacy switch. Thus, substituting N114 with an alanine (N114A) completely abolishes Gαi activation by 7α,25-OHC even though the specific binding of the [(3) H]-7α,25-OHC radioligand increases. In contrast, the N114A mutant is still able to recruit β-arrestin and even with enhanced the potency (18.7-fold) compared to EBI2 WT. Underscoring the key role of N114, we also show that sodium has an negative allosteric effect on oxysterol binding and that this is mediated via N114. This is further supported by molecular modelling of the ion binding site based on a EBI2 homology model. Conclusions and Implications Collectively, our data points to N114 as a key residue for EBI2 signaling controlling the balance between G protein-dependent and -independent pathways and facilitating sodium binding.

U2 - 10.1111/bph.13801

DO - 10.1111/bph.13801

M3 - Journal article

C2 - 28369721

VL - 174

SP - 2031

EP - 2042

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 13

ER -

ID: 176653079