Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics. / Nikolajsen, Rikke P; Hansen, Åse Marie; Bro, R.
I: Luminescence, Bind 16, Nr. 2, 2000, s. 91-101.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Attempt to separate the fluorescence spectra of adrenaline and noradrenaline using chemometrics
AU - Nikolajsen, Rikke P
AU - Hansen, Åse Marie
AU - Bro, R
N1 - Copyright 2001 John Wiley & Sons, Ltd.
PY - 2000
Y1 - 2000
N2 - An investigation was conducted on whether the fluorescence spectra of the very similar catecholamines adrenaline and noradrenaline could be separated using chemometric methods. The fluorescence landscapes (several excitation and emission spectra were measured) of two data sets with respectively 16 and 6 samples were measured, the smaller data set with higher resolution and i.e. precision. The samples were artificial urine (pH approximately equal to 3) spiked with the catecholamines in the concentration ranges 40--1200 nmol/L and 5.5--18 micromol/L, respectively. Unfold partial least squares regression (Unfold-PLSR) on the larger data set and parallel factor analysis (PARAFAC) of the six samples of the smaller set showed that there was no difference between the fluorescence landscapes of adrenaline and noradrenaline. It can be concluded that chemometric separation of adrenaline and noradrenaline is not obtainable using this type of fluorescence measurement. Raman scatter, which overlaps the catecholamine spectra, was shown not to have any influence on the models calculated.
AB - An investigation was conducted on whether the fluorescence spectra of the very similar catecholamines adrenaline and noradrenaline could be separated using chemometric methods. The fluorescence landscapes (several excitation and emission spectra were measured) of two data sets with respectively 16 and 6 samples were measured, the smaller data set with higher resolution and i.e. precision. The samples were artificial urine (pH approximately equal to 3) spiked with the catecholamines in the concentration ranges 40--1200 nmol/L and 5.5--18 micromol/L, respectively. Unfold partial least squares regression (Unfold-PLSR) on the larger data set and parallel factor analysis (PARAFAC) of the six samples of the smaller set showed that there was no difference between the fluorescence landscapes of adrenaline and noradrenaline. It can be concluded that chemometric separation of adrenaline and noradrenaline is not obtainable using this type of fluorescence measurement. Raman scatter, which overlaps the catecholamine spectra, was shown not to have any influence on the models calculated.
KW - Calibration
KW - Epinephrine
KW - Multivariate Analysis
KW - Norepinephrine
KW - Spectrometry, Fluorescence
U2 - 10.1002/bio.637
DO - 10.1002/bio.637
M3 - Journal article
C2 - 11312534
VL - 16
SP - 91
EP - 101
JO - Luminescence
JF - Luminescence
SN - 1522-7235
IS - 2
ER -
ID: 48865608