AtRsgA from Arabidopsis thaliana is important for maturation of the small subunit of the chloroplast ribosome
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AtRsgA from Arabidopsis thaliana is important for maturation of the small subunit of the chloroplast ribosome. / Janowski, Marcin; Zoschke, Reimo; Scharff, Lars; Martinez Jaime, Silvia; Ferrari, Camilla; Proost, Sebastian; Ng Wei Xiong, Jonathan; Omranian, Nooshin; Musialak-Lange, Magdalena; Nikoloski, Zoran; Graf, Alexander; Schöttler, Mark A.; Sampathkumar, Arun; Vaid, Neha; Mutwil, Marek.
I: The Plant Journal, Bind 96, Nr. 2, 2018, s. 404-420.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - AtRsgA from Arabidopsis thaliana is important for maturation of the small subunit of the chloroplast ribosome
AU - Janowski, Marcin
AU - Zoschke, Reimo
AU - Scharff, Lars
AU - Martinez Jaime, Silvia
AU - Ferrari, Camilla
AU - Proost, Sebastian
AU - Ng Wei Xiong, Jonathan
AU - Omranian, Nooshin
AU - Musialak-Lange, Magdalena
AU - Nikoloski, Zoran
AU - Graf, Alexander
AU - Schöttler, Mark A.
AU - Sampathkumar, Arun
AU - Vaid, Neha
AU - Mutwil, Marek
PY - 2018
Y1 - 2018
N2 - Plastid ribosomes are very similar in structure and function to ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favourable at biological conditions, it requires activity of many assembly factors. Here, we have characterized a homolog of bacterial rsgA in Arabidopsis thaliana and show that it can complement the bacterial homolog. Functional characterization of a strong mutant in Arabidopsis revealed that the protein is essential for plant viability, while a weak mutant produced dwarf, chlorotic plants that incorporated immature pre-16S ribosomal RNA into translating ribosomes. Physiological analysis of the mutant plants revealed smaller, but more numerous chloroplasts in the mesophyll cells, reduction of chlorophyll a and b, depletion of proplastids from the rib meristem and decreased photosynthetic electron transport rate and efficiency. Comparative RNA-sequencing and proteomic analysis of the weak mutant and wild-type plants revealed that various biotic stress-related, transcriptional regulation and post-transcriptional modification pathways were repressed in the mutant. Intriguingly, while nuclear- and chloroplast-encoded photosynthesis-related proteins were less abundant in the mutant, the corresponding transcripts were increased, suggesting an elaborate compensatory mechanism, potentially via differentially active retrograde signalling pathways. To conclude, this study reveals a chloroplast ribosome assembly factor and outlines the transcriptomic and proteomic responses of the compensatory mechanism activated during decreased chloroplast function.
AB - Plastid ribosomes are very similar in structure and function to ribosomes of their bacterial ancestors. Since ribosome biogenesis is not thermodynamically favourable at biological conditions, it requires activity of many assembly factors. Here, we have characterized a homolog of bacterial rsgA in Arabidopsis thaliana and show that it can complement the bacterial homolog. Functional characterization of a strong mutant in Arabidopsis revealed that the protein is essential for plant viability, while a weak mutant produced dwarf, chlorotic plants that incorporated immature pre-16S ribosomal RNA into translating ribosomes. Physiological analysis of the mutant plants revealed smaller, but more numerous chloroplasts in the mesophyll cells, reduction of chlorophyll a and b, depletion of proplastids from the rib meristem and decreased photosynthetic electron transport rate and efficiency. Comparative RNA-sequencing and proteomic analysis of the weak mutant and wild-type plants revealed that various biotic stress-related, transcriptional regulation and post-transcriptional modification pathways were repressed in the mutant. Intriguingly, while nuclear- and chloroplast-encoded photosynthesis-related proteins were less abundant in the mutant, the corresponding transcripts were increased, suggesting an elaborate compensatory mechanism, potentially via differentially active retrograde signalling pathways. To conclude, this study reveals a chloroplast ribosome assembly factor and outlines the transcriptomic and proteomic responses of the compensatory mechanism activated during decreased chloroplast function.
KW - 30s subunit
KW - arabidopsis thaliana
KW - assembly factor
KW - chloroplast ribosome
KW - corresponding authors
KW - ribosome assembly
KW - rsga
U2 - 10.1111/tpj.14040
DO - 10.1111/tpj.14040
M3 - Journal article
C2 - 30044525
VL - 96
SP - 404
EP - 420
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 2
ER -
ID: 200139338