Antidiabetic constituents of Dendrobium officinale as determined by high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition combined with HPLC-PDA-HRMS-SPE-NMR analysis.
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Antidiabetic constituents of Dendrobium officinale as determined by high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition combined with HPLC-PDA-HRMS-SPE-NMR analysis. / Chu, Chu; Li, Tuo; Pedersen, Hans Albert; Kongstad, Kenneth Thermann; Yan, Jizhong; Stærk, Dan.
I: Phytochemistry Letters, Bind 31, 2019, s. 47-52.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Antidiabetic constituents of Dendrobium officinale as determined by high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition combined with HPLC-PDA-HRMS-SPE-NMR analysis.
AU - Chu, Chu
AU - Li, Tuo
AU - Pedersen, Hans Albert
AU - Kongstad, Kenneth Thermann
AU - Yan, Jizhong
AU - Stærk, Dan
PY - 2019
Y1 - 2019
N2 - Type 2 diabetes is a chronic metabolic disorder affecting millions of people worldwide, and new drug leads are urgently needed. The dried stem of Dendrobium officinale is used in China to alleviate the symptoms of diabetes, but investigations of its bioactive components have been limited. We have applied a simple, fast, and effective method, combining triple high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition with high-performance liquid chromatography - photodiode-array detection - high-resolution mass spectrometry - solid-phase extraction - nuclear magnetic resonance spectroscopy (HR-bioassay/HPLC-PDA-HRMS-SPE-NMR). Two new compounds, 3,4,4′-trihydroxy-5-methoxybibenzyl (5) and 3,4-dihydroxy-3′,4′,5-trimethoxybibenzyl (8a), along with fourteen known compounds, were identified. Twelve of these were associated with radical scavenging activity, six with α-glucosidase inhibition, and one with α-amylase inhibition. Compound 5, dendrocandin U, and 3,4-dihydroxy-4′,5-dimethoxybibenzyl (12) are reported as α-glucosidase inhibitors for the first time, and 12 is furthermore reported as an α-amylase inhibitor for the first time
AB - Type 2 diabetes is a chronic metabolic disorder affecting millions of people worldwide, and new drug leads are urgently needed. The dried stem of Dendrobium officinale is used in China to alleviate the symptoms of diabetes, but investigations of its bioactive components have been limited. We have applied a simple, fast, and effective method, combining triple high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition with high-performance liquid chromatography - photodiode-array detection - high-resolution mass spectrometry - solid-phase extraction - nuclear magnetic resonance spectroscopy (HR-bioassay/HPLC-PDA-HRMS-SPE-NMR). Two new compounds, 3,4,4′-trihydroxy-5-methoxybibenzyl (5) and 3,4-dihydroxy-3′,4′,5-trimethoxybibenzyl (8a), along with fourteen known compounds, were identified. Twelve of these were associated with radical scavenging activity, six with α-glucosidase inhibition, and one with α-amylase inhibition. Compound 5, dendrocandin U, and 3,4-dihydroxy-4′,5-dimethoxybibenzyl (12) are reported as α-glucosidase inhibitors for the first time, and 12 is furthermore reported as an α-amylase inhibitor for the first time
U2 - 10.1016/j.phytol.2019.03.002
DO - 10.1016/j.phytol.2019.03.002
M3 - Journal article
VL - 31
SP - 47
EP - 52
JO - Phytochemistry Letters
JF - Phytochemistry Letters
SN - 1874-3900
ER -
ID: 213550593