Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids: Mammalian embryonic organoids

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Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids : Mammalian embryonic organoids. / Turner, David A.; Girgin, Mehmet; Alonso-Crisostomo, Luz; Trivedi, Vikas; Baillie-Johnson, Peter; Glodowski, Cherise R.; Hayward, Penelope C.; Collignon, Jérôme; Gustavsen, Carsten; Serup, Palle; Steventon, Benjamin; Lutolf, Matthias P.; Arias, Alfonso Martinez.

I: Development (Cambridge), Bind 144, Nr. 21, 11.2017, s. 3894-3906.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Turner, DA, Girgin, M, Alonso-Crisostomo, L, Trivedi, V, Baillie-Johnson, P, Glodowski, CR, Hayward, PC, Collignon, J, Gustavsen, C, Serup, P, Steventon, B, Lutolf, MP & Arias, AM 2017, 'Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids: Mammalian embryonic organoids', Development (Cambridge), bind 144, nr. 21, s. 3894-3906. https://doi.org/10.1242/dev.150391

APA

Turner, D. A., Girgin, M., Alonso-Crisostomo, L., Trivedi, V., Baillie-Johnson, P., Glodowski, C. R., Hayward, P. C., Collignon, J., Gustavsen, C., Serup, P., Steventon, B., Lutolf, M. P., & Arias, A. M. (2017). Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids: Mammalian embryonic organoids. Development (Cambridge), 144(21), 3894-3906. https://doi.org/10.1242/dev.150391

Vancouver

Turner DA, Girgin M, Alonso-Crisostomo L, Trivedi V, Baillie-Johnson P, Glodowski CR o.a. Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids: Mammalian embryonic organoids. Development (Cambridge). 2017 nov.;144(21):3894-3906. https://doi.org/10.1242/dev.150391

Author

Turner, David A. ; Girgin, Mehmet ; Alonso-Crisostomo, Luz ; Trivedi, Vikas ; Baillie-Johnson, Peter ; Glodowski, Cherise R. ; Hayward, Penelope C. ; Collignon, Jérôme ; Gustavsen, Carsten ; Serup, Palle ; Steventon, Benjamin ; Lutolf, Matthias P. ; Arias, Alfonso Martinez. / Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids : Mammalian embryonic organoids. I: Development (Cambridge). 2017 ; Bind 144, Nr. 21. s. 3894-3906.

Bibtex

@article{5d769d38c2924d3c91b19d198ebced85,
title = "Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids: Mammalian embryonic organoids",
abstract = "The establishment of the anteroposterior (AP) axis is a crucial step during animal embryo development. In mammals, genetic studies have shown that this process relies on signals spatiotemporally deployed in the extra-embryonic tissues that locate the position of the head and the onset of gastrulation, marked by T/Brachyury (T/Bra) at the posterior of the embryo. Here, we use gastruloids, mESC-based organoids, as a model system with which to study this process. We find that gastruloids localise T/Bra expression to one end and undergo elongation similar to the posterior region of the embryo, suggesting that they develop an AP axis. This process relies on precisely timed interactions between Wnt/β-catenin and Nodal signalling, whereas BMP signalling is dispensable. Additionally, polarised T/Bra expression occurs in the absence of extra-embryonic tissues or localised sources of signals. We suggest that the role of extra-embryonic tissues in the mammalian embryo might not be to induce the axes but to bias an intrinsic ability of the embryo to initially break symmetry. Furthermore, we suggest that Wnt signalling has a separable activity involved in the elongation of the axis.",
keywords = "Axial organisation, Gastruloids, Organoids, Symmetry-breaking",
author = "Turner, {David A.} and Mehmet Girgin and Luz Alonso-Crisostomo and Vikas Trivedi and Peter Baillie-Johnson and Glodowski, {Cherise R.} and Hayward, {Penelope C.} and J{\'e}r{\^o}me Collignon and Carsten Gustavsen and Palle Serup and Benjamin Steventon and Lutolf, {Matthias P.} and Arias, {Alfonso Martinez}",
year = "2017",
month = nov,
doi = "10.1242/dev.150391",
language = "English",
volume = "144",
pages = "3894--3906",
journal = "Development",
issn = "0950-1991",
publisher = "The Company of Biologists",
number = "21",

}

RIS

TY - JOUR

T1 - Anteroposterior polarity and elongation in the absence of extraembryonic tissues and of spatially localised signalling in gastruloids

T2 - Mammalian embryonic organoids

AU - Turner, David A.

AU - Girgin, Mehmet

AU - Alonso-Crisostomo, Luz

AU - Trivedi, Vikas

AU - Baillie-Johnson, Peter

AU - Glodowski, Cherise R.

AU - Hayward, Penelope C.

AU - Collignon, Jérôme

AU - Gustavsen, Carsten

AU - Serup, Palle

AU - Steventon, Benjamin

AU - Lutolf, Matthias P.

AU - Arias, Alfonso Martinez

PY - 2017/11

Y1 - 2017/11

N2 - The establishment of the anteroposterior (AP) axis is a crucial step during animal embryo development. In mammals, genetic studies have shown that this process relies on signals spatiotemporally deployed in the extra-embryonic tissues that locate the position of the head and the onset of gastrulation, marked by T/Brachyury (T/Bra) at the posterior of the embryo. Here, we use gastruloids, mESC-based organoids, as a model system with which to study this process. We find that gastruloids localise T/Bra expression to one end and undergo elongation similar to the posterior region of the embryo, suggesting that they develop an AP axis. This process relies on precisely timed interactions between Wnt/β-catenin and Nodal signalling, whereas BMP signalling is dispensable. Additionally, polarised T/Bra expression occurs in the absence of extra-embryonic tissues or localised sources of signals. We suggest that the role of extra-embryonic tissues in the mammalian embryo might not be to induce the axes but to bias an intrinsic ability of the embryo to initially break symmetry. Furthermore, we suggest that Wnt signalling has a separable activity involved in the elongation of the axis.

AB - The establishment of the anteroposterior (AP) axis is a crucial step during animal embryo development. In mammals, genetic studies have shown that this process relies on signals spatiotemporally deployed in the extra-embryonic tissues that locate the position of the head and the onset of gastrulation, marked by T/Brachyury (T/Bra) at the posterior of the embryo. Here, we use gastruloids, mESC-based organoids, as a model system with which to study this process. We find that gastruloids localise T/Bra expression to one end and undergo elongation similar to the posterior region of the embryo, suggesting that they develop an AP axis. This process relies on precisely timed interactions between Wnt/β-catenin and Nodal signalling, whereas BMP signalling is dispensable. Additionally, polarised T/Bra expression occurs in the absence of extra-embryonic tissues or localised sources of signals. We suggest that the role of extra-embryonic tissues in the mammalian embryo might not be to induce the axes but to bias an intrinsic ability of the embryo to initially break symmetry. Furthermore, we suggest that Wnt signalling has a separable activity involved in the elongation of the axis.

KW - Axial organisation

KW - Gastruloids

KW - Organoids

KW - Symmetry-breaking

U2 - 10.1242/dev.150391

DO - 10.1242/dev.150391

M3 - Journal article

C2 - 28951435

AN - SCOPUS:85032790109

VL - 144

SP - 3894

EP - 3906

JO - Development

JF - Development

SN - 0950-1991

IS - 21

ER -

ID: 196441673