Annotation of the protein coding regions of the equine genome

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Standard

Annotation of the protein coding regions of the equine genome. / Hestand, Matthew S.; Kalbfleisch, Theodore S.; Coleman, Stephen J.; Zeng, Zheng; Liu, Jinze; Orlando, Ludovic Antoine Alexandre; MacLeod, James N.

I: PLOS ONE, Bind 10, Nr. 6, e0124375, 2015.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Hestand, MS, Kalbfleisch, TS, Coleman, SJ, Zeng, Z, Liu, J, Orlando, LAA & MacLeod, JN 2015, 'Annotation of the protein coding regions of the equine genome', PLOS ONE, bind 10, nr. 6, e0124375. https://doi.org/10.1371/journal.pone.0124375

APA

Hestand, M. S., Kalbfleisch, T. S., Coleman, S. J., Zeng, Z., Liu, J., Orlando, L. A. A., & MacLeod, J. N. (2015). Annotation of the protein coding regions of the equine genome. PLOS ONE, 10(6), [e0124375]. https://doi.org/10.1371/journal.pone.0124375

Vancouver

Hestand MS, Kalbfleisch TS, Coleman SJ, Zeng Z, Liu J, Orlando LAA o.a. Annotation of the protein coding regions of the equine genome. PLOS ONE. 2015;10(6). e0124375. https://doi.org/10.1371/journal.pone.0124375

Author

Hestand, Matthew S. ; Kalbfleisch, Theodore S. ; Coleman, Stephen J. ; Zeng, Zheng ; Liu, Jinze ; Orlando, Ludovic Antoine Alexandre ; MacLeod, James N. / Annotation of the protein coding regions of the equine genome. I: PLOS ONE. 2015 ; Bind 10, Nr. 6.

Bibtex

@article{55bec5ae5ddf419fa54f7b39be5a118d,
title = "Annotation of the protein coding regions of the equine genome",
abstract = "Current gene annotation of the horse genome is largely derived from in silico predictions and cross-species alignments. Only a small number of genes are annotated based on equine EST and mRNA sequences. To expand the number of equine genes annotated from equine experimental evidence, we sequenced mRNA from a pool of forty-three different tissues. From these, we derived the structures of 68,594 transcripts. In addition, we identified 301,829 positions with SNPs or small indels within these transcripts relative to EquCab2. Interestingly, 780 variants extend the open reading frame of the transcript and appear to be small errors in the equine reference genome, since they are also identified as homozygous variants by genomic DNA resequencing of the reference horse. Taken together, we provide a resource of equine mRNA structures and protein coding variants that will enhance equine and cross-species transcriptional and genomic comparisons.",
author = "Hestand, {Matthew S.} and Kalbfleisch, {Theodore S.} and Coleman, {Stephen J.} and Zheng Zeng and Jinze Liu and Orlando, {Ludovic Antoine Alexandre} and MacLeod, {James N.}",
year = "2015",
doi = "10.1371/journal.pone.0124375",
language = "English",
volume = "10",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - Annotation of the protein coding regions of the equine genome

AU - Hestand, Matthew S.

AU - Kalbfleisch, Theodore S.

AU - Coleman, Stephen J.

AU - Zeng, Zheng

AU - Liu, Jinze

AU - Orlando, Ludovic Antoine Alexandre

AU - MacLeod, James N.

PY - 2015

Y1 - 2015

N2 - Current gene annotation of the horse genome is largely derived from in silico predictions and cross-species alignments. Only a small number of genes are annotated based on equine EST and mRNA sequences. To expand the number of equine genes annotated from equine experimental evidence, we sequenced mRNA from a pool of forty-three different tissues. From these, we derived the structures of 68,594 transcripts. In addition, we identified 301,829 positions with SNPs or small indels within these transcripts relative to EquCab2. Interestingly, 780 variants extend the open reading frame of the transcript and appear to be small errors in the equine reference genome, since they are also identified as homozygous variants by genomic DNA resequencing of the reference horse. Taken together, we provide a resource of equine mRNA structures and protein coding variants that will enhance equine and cross-species transcriptional and genomic comparisons.

AB - Current gene annotation of the horse genome is largely derived from in silico predictions and cross-species alignments. Only a small number of genes are annotated based on equine EST and mRNA sequences. To expand the number of equine genes annotated from equine experimental evidence, we sequenced mRNA from a pool of forty-three different tissues. From these, we derived the structures of 68,594 transcripts. In addition, we identified 301,829 positions with SNPs or small indels within these transcripts relative to EquCab2. Interestingly, 780 variants extend the open reading frame of the transcript and appear to be small errors in the equine reference genome, since they are also identified as homozygous variants by genomic DNA resequencing of the reference horse. Taken together, we provide a resource of equine mRNA structures and protein coding variants that will enhance equine and cross-species transcriptional and genomic comparisons.

U2 - 10.1371/journal.pone.0124375

DO - 10.1371/journal.pone.0124375

M3 - Journal article

C2 - 26107351

AN - SCOPUS:84939213635

VL - 10

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

M1 - e0124375

ER -

ID: 154399010