Ancient DNA preserved in small bone fragments from the PW Lund collection
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Ancient DNA preserved in small bone fragments from the PW Lund collection. / Seersholm, Frederik; Hansen, Kasper Lykke; Heydenrych, Matthew; Hansen, Anders J.; Bunce, Michael; Allentoft, Morten E.
I: Ecology and Evolution, Bind 11, Nr. 5, 2021, s. 2064-2071.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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T1 - Ancient DNA preserved in small bone fragments from the PW Lund collection
AU - Seersholm, Frederik
AU - Hansen, Kasper Lykke
AU - Heydenrych, Matthew
AU - Hansen, Anders J.
AU - Bunce, Michael
AU - Allentoft, Morten E.
PY - 2021
Y1 - 2021
N2 - The Lund collection is one of the oldest subfossil collections in the world. The vast assemblage of subfossils was collected in the 1830s and 1840s by Peter Wilhelm Lund in Lagoa Santa, Brazil, and was shipped to Copenhagen in 1848, where it was stored in various locations around the city with little attention for the future preservation of the collection. So far, successful genetic research on the material collected by Lund has been limited to two samples of human petrous bone. However, less is known about the preservation conditions of the vast amounts of small and fragmentary bones stored in the collection. To address this, we studied ancient DNA from bulk bone samples of approximately 100 bone fragments from the P.W. Lund collection from boxes with varying degrees of physical preservation conditions. Using bulk bone metabarcoding, we found a high species diversity in all samples. In total, we identified 17 species, representing 11 mammals, two birds, one fish, and three frogs. Of these, two species are new to the collection. Collectively, these results exhibit the potential of future genetic studies on the famous P.W. Lund collection and suggest that the effects of poor storage conditions are probably negligible compared with the long-term in situ degradation that specimens undergo before excavation.
AB - The Lund collection is one of the oldest subfossil collections in the world. The vast assemblage of subfossils was collected in the 1830s and 1840s by Peter Wilhelm Lund in Lagoa Santa, Brazil, and was shipped to Copenhagen in 1848, where it was stored in various locations around the city with little attention for the future preservation of the collection. So far, successful genetic research on the material collected by Lund has been limited to two samples of human petrous bone. However, less is known about the preservation conditions of the vast amounts of small and fragmentary bones stored in the collection. To address this, we studied ancient DNA from bulk bone samples of approximately 100 bone fragments from the P.W. Lund collection from boxes with varying degrees of physical preservation conditions. Using bulk bone metabarcoding, we found a high species diversity in all samples. In total, we identified 17 species, representing 11 mammals, two birds, one fish, and three frogs. Of these, two species are new to the collection. Collectively, these results exhibit the potential of future genetic studies on the famous P.W. Lund collection and suggest that the effects of poor storage conditions are probably negligible compared with the long-term in situ degradation that specimens undergo before excavation.
KW - ancient DNA
KW - bulk bone
KW - metabarcoding
KW - museum collections
KW - palaeontology
U2 - 10.1002/ece3.7162
DO - 10.1002/ece3.7162
M3 - Journal article
C2 - 33717442
VL - 11
SP - 2064
EP - 2071
JO - Ecology and Evolution
JF - Ecology and Evolution
SN - 2045-7758
IS - 5
ER -
ID: 261070062