An Unexpected Location of the Arginine Catabolic Mobile Element (ACME) in a USA300-Related MRSA Strain
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An Unexpected Location of the Arginine Catabolic Mobile Element (ACME) in a USA300-Related MRSA Strain. / Bartels, Mette Damkjær; Hansen, Lars H.; Boye, Kit; Sørensen, Søren Johannes; Westh, Henrik T.
I: P L o S One, Bind 6, Nr. 1, e16193, 2011.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - An Unexpected Location of the Arginine Catabolic Mobile Element (ACME) in a USA300-Related MRSA Strain
AU - Bartels, Mette Damkjær
AU - Hansen, Lars H.
AU - Boye, Kit
AU - Sørensen, Søren Johannes
AU - Westh, Henrik T.
PY - 2011
Y1 - 2011
N2 - In methicillin resistant Staphylococcus aureus (MRSA), the arginine catabolic mobile element (ACME) was initially describedin USA300 (t008-ST8) where it is located downstream of the staphylococcal cassette chromosome mec (SCCmec). A commonhealth-care associated MRSA in Copenhagen, Denmark (t024-ST8) is clonally related to USA300 and is frequently PCRpositive for the ACME specific arcA-gene. This study is the first to describe an ACME element upstream of the SCCmec inMRSA. By traditional SCCmec typing schemes, the SCCmec of t024-ST8 strain M1 carries SCCmec IVa, but full sequencing ofthe cassette revealed that the entire J3 region had no homology to published SCCmec IVa. Within the J3 region of M1 was a1705 bp sequence only similar to a sequence in S. haemolyticus strain JCSC1435 and 2941 bps with no homology found inGenBank. In addition to the usual direct repeats (DR) at each extremity of SCCmec, M1 had two new DR between the orfXgene and the J3 region of the SCCmec. The region between the orfX DR (DR1) and DR2 contained the ccrAB4 genes. AnACME II-like element was located between DR2 and DR3. The entire 26,468 bp sequence between DR1 and DR3 was highlysimilar to parts of the ACME composite island of S. epidermidis strain ATCC12228. Sequencing of an ACME negative t024-ST8strain (M299) showed that DR1 and the sequence between DR1 and DR3 was missing. The finding of a mobile ACME II-likeelement inserted downstream of orfX and upstream of SCCmec indicates a novel recombination between staphylococcalspecies.
AB - In methicillin resistant Staphylococcus aureus (MRSA), the arginine catabolic mobile element (ACME) was initially describedin USA300 (t008-ST8) where it is located downstream of the staphylococcal cassette chromosome mec (SCCmec). A commonhealth-care associated MRSA in Copenhagen, Denmark (t024-ST8) is clonally related to USA300 and is frequently PCRpositive for the ACME specific arcA-gene. This study is the first to describe an ACME element upstream of the SCCmec inMRSA. By traditional SCCmec typing schemes, the SCCmec of t024-ST8 strain M1 carries SCCmec IVa, but full sequencing ofthe cassette revealed that the entire J3 region had no homology to published SCCmec IVa. Within the J3 region of M1 was a1705 bp sequence only similar to a sequence in S. haemolyticus strain JCSC1435 and 2941 bps with no homology found inGenBank. In addition to the usual direct repeats (DR) at each extremity of SCCmec, M1 had two new DR between the orfXgene and the J3 region of the SCCmec. The region between the orfX DR (DR1) and DR2 contained the ccrAB4 genes. AnACME II-like element was located between DR2 and DR3. The entire 26,468 bp sequence between DR1 and DR3 was highlysimilar to parts of the ACME composite island of S. epidermidis strain ATCC12228. Sequencing of an ACME negative t024-ST8strain (M299) showed that DR1 and the sequence between DR1 and DR3 was missing. The finding of a mobile ACME II-likeelement inserted downstream of orfX and upstream of SCCmec indicates a novel recombination between staphylococcalspecies.
U2 - 10.1371/journal.pone.0016193
DO - 10.1371/journal.pone.0016193
M3 - Journal article
C2 - 21283578
VL - 6
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 1
M1 - e16193
ER -
ID: 33533250