An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells
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An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells. / Narimatsu, Yoshiki; Joshi, Hiren J.; Nason, Rebecca; Van Coillie, Julie; Karlsson, Richard; Sun, Lingbo; Ye, Zilu; Chen, Yen-Hsi; Schjoldager, Katrine T.; Steentoft, Catharina; Furukawa, Sanae; Bensing, Barbara A.; Sullam, Paul M.; Thompson, Andrew J.; Paulson, James C.; Büll, Christian; Adema, Gosse J.; Mandel, Ulla; Hansen, Lars; Bennett, Eric Paul; Varki, Ajit; Vakhrushev, Sergey Y.; Yang, Zhang; Clausen, Henrik.
I: Molecular Cell, Bind 75, Nr. 2, 2019, s. 394-407, e1-e5.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells
AU - Narimatsu, Yoshiki
AU - Joshi, Hiren J.
AU - Nason, Rebecca
AU - Van Coillie, Julie
AU - Karlsson, Richard
AU - Sun, Lingbo
AU - Ye, Zilu
AU - Chen, Yen-Hsi
AU - Schjoldager, Katrine T.
AU - Steentoft, Catharina
AU - Furukawa, Sanae
AU - Bensing, Barbara A.
AU - Sullam, Paul M.
AU - Thompson, Andrew J.
AU - Paulson, James C.
AU - Büll, Christian
AU - Adema, Gosse J.
AU - Mandel, Ulla
AU - Hansen, Lars
AU - Bennett, Eric Paul
AU - Varki, Ajit
AU - Vakhrushev, Sergey Y.
AU - Yang, Zhang
AU - Clausen, Henrik
N1 - Copyright © 2019 Elsevier Inc. All rights reserved.
PY - 2019
Y1 - 2019
N2 - The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.
AB - The structural diversity of glycans on cells-the glycome-is vast and complex to decipher. Glycan arrays display oligosaccharides and are used to report glycan hapten binding epitopes. Glycan arrays are limited resources and present saccharides without the context of other glycans and glycoconjugates. We used maps of glycosylation pathways to generate a library of isogenic HEK293 cells with combinatorially engineered glycosylation capacities designed to display and dissect the genetic, biosynthetic, and structural basis for glycan binding in a natural context. The cell-based glycan array is self-renewable and reports glycosyltransferase genes required (or blocking) for interactions through logical sequential biosynthetic steps, which is predictive of structural glycan features involved and provides instructions for synthesis, recombinant production, and genetic dissection strategies. Broad utility of the cell-based glycan array is demonstrated, and we uncover higher order binding of microbial adhesins to clustered patches of O-glycans organized by their presentation on proteins.
U2 - 10.1016/j.molcel.2019.05.017
DO - 10.1016/j.molcel.2019.05.017
M3 - Journal article
C2 - 31227230
VL - 75
SP - 394-407, e1-e5
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 2
ER -
ID: 222922957