An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

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  • Liaoyuan Zhang
  • Singh, Raushan Kumar
  • D. Sivakumar
  • Zewang Guo
  • Jiahuan Li
  • Fanbing Chen
  • Yuanzhi He
  • Xiong Guan
  • Yun Chan Kang
  • Jung Kul Lee

Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

TidsskriftGreen Chemistry
Udgave nummer1
Sider (fra-til)230-242
Antal sider13
StatusUdgivet - 1 jan. 2018

ID: 229900493