An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

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An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis. / Zhang, Liaoyuan; Singh, Raushan; Sivakumar, D.; Guo, Zewang; Li, Jiahuan; Chen, Fanbing; He, Yuanzhi; Guan, Xiong; Kang, Yun Chan; Lee, Jung Kul.

I: Green Chemistry, Bind 20, Nr. 1, 01.01.2018, s. 230-242.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Zhang, L, Singh, R, Sivakumar, D, Guo, Z, Li, J, Chen, F, He, Y, Guan, X, Kang, YC & Lee, JK 2018, 'An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis', Green Chemistry, bind 20, nr. 1, s. 230-242. https://doi.org/10.1039/c7gc02898a

APA

Zhang, L., Singh, R., Sivakumar, D., Guo, Z., Li, J., Chen, F., He, Y., Guan, X., Kang, Y. C., & Lee, J. K. (2018). An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis. Green Chemistry, 20(1), 230-242. https://doi.org/10.1039/c7gc02898a

Vancouver

Zhang L, Singh R, Sivakumar D, Guo Z, Li J, Chen F o.a. An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis. Green Chemistry. 2018 jan. 1;20(1):230-242. https://doi.org/10.1039/c7gc02898a

Author

Zhang, Liaoyuan ; Singh, Raushan ; Sivakumar, D. ; Guo, Zewang ; Li, Jiahuan ; Chen, Fanbing ; He, Yuanzhi ; Guan, Xiong ; Kang, Yun Chan ; Lee, Jung Kul. / An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis. I: Green Chemistry. 2018 ; Bind 20, Nr. 1. s. 230-242.

Bibtex

@article{c6c2ae83b7fd4ab28caf070bfb8a5866,

title = "An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis",

abstract = "Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.",

author = "Liaoyuan Zhang and Raushan Singh and D. Sivakumar and Zewang Guo and Jiahuan Li and Fanbing Chen and Yuanzhi He and Xiong Guan and Kang, {Yun Chan} and Lee, {Jung Kul}",

year = "2018",

month = jan,

day = "1",

doi = "10.1039/c7gc02898a",

language = "English",

volume = "20",

pages = "230--242",

journal = "Green Chemistry",

issn = "1463-9262",

publisher = "Royal Society of Chemistry",

number = "1",

}

RIS

TY - JOUR

T1 - An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis

AU - Zhang, Liaoyuan

AU - Singh, Raushan

AU - Sivakumar, D.

AU - Guo, Zewang

AU - Li, Jiahuan

AU - Chen, Fanbing

AU - He, Yuanzhi

AU - Guan, Xiong

AU - Kang, Yun Chan

AU - Lee, Jung Kul

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

AB - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.

U2 - 10.1039/c7gc02898a

DO - 10.1039/c7gc02898a

M3 - Journal article

AN - SCOPUS:85040191293

VL - 20

SP - 230

EP - 242

JO - Green Chemistry

JF - Green Chemistry

SN - 1463-9262

IS - 1

ER -

ID: 229900493