AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Dokumenter

  • Marc Dohmen
  • Sarah Krieg
  • Georgios Agalaridis
  • Xiaoqing Zhu
  • Saifeldin N Shehata
  • Elisabeth Pfeiffenberger
  • Jan Amelang
  • Mareike Bütepage
  • Elena Buerova
  • Carolina M Pfaff
  • Dipanjan Chanda
  • Stephan Geley
  • Christian Preisinger
  • Sakamoto, Kei
  • Bernhard Lüscher
  • Dietbert Neumann
  • Jörg Vervoorts

The AMP-activated protein kinase (AMPK) is a master sensor of the cellular energy status that is crucial for the adaptive response to limited energy availability. AMPK is implicated in the regulation of many cellular processes, including autophagy. However, the precise mechanisms by which AMPK controls these processes and the identities of relevant substrates are not fully understood. Using protein microarrays, we identify Cyclin Y as an AMPK substrate that is phosphorylated at Serine 326 (S326) both in vitro and in cells. Phosphorylation of Cyclin Y at S326 promotes its interaction with the Cyclin-dependent kinase 16 (CDK16), thereby stimulating its catalytic activity. When expressed in cells, Cyclin Y/CDK16 is sufficient to promote autophagy. Moreover, Cyclin Y/CDK16 is necessary for efficient AMPK-dependent activation of autophagy. This functional interaction is mediated by AMPK phosphorylating S326 of Cyclin Y. Collectively, we define Cyclin Y/CDK16 as downstream effector of AMPK for inducing autophagy.

OriginalsprogEngelsk
Artikelnummer1032
TidsskriftNature Communications
Vol/bind11
Udgave nummer1
Antal sider18
ISSN2041-1723
DOI
StatusUdgivet - 2020

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