AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy

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AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. / Dohmen, Marc; Krieg, Sarah; Agalaridis, Georgios; Zhu, Xiaoqing; Shehata, Saifeldin N; Pfeiffenberger, Elisabeth; Amelang, Jan; Bütepage, Mareike; Buerova, Elena; Pfaff, Carolina M; Chanda, Dipanjan; Geley, Stephan; Preisinger, Christian; Sakamoto, Kei; Lüscher, Bernhard; Neumann, Dietbert; Vervoorts, Jörg.

I: Nature Communications, Bind 11, Nr. 1, 1032, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Dohmen, M, Krieg, S, Agalaridis, G, Zhu, X, Shehata, SN, Pfeiffenberger, E, Amelang, J, Bütepage, M, Buerova, E, Pfaff, CM, Chanda, D, Geley, S, Preisinger, C, Sakamoto, K, Lüscher, B, Neumann, D & Vervoorts, J 2020, 'AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy', Nature Communications, bind 11, nr. 1, 1032. https://doi.org/10.1038/s41467-020-14812-0

APA

Dohmen, M., Krieg, S., Agalaridis, G., Zhu, X., Shehata, S. N., Pfeiffenberger, E., Amelang, J., Bütepage, M., Buerova, E., Pfaff, C. M., Chanda, D., Geley, S., Preisinger, C., Sakamoto, K., Lüscher, B., Neumann, D., & Vervoorts, J. (2020). AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nature Communications, 11(1), [1032]. https://doi.org/10.1038/s41467-020-14812-0

Vancouver

Dohmen M, Krieg S, Agalaridis G, Zhu X, Shehata SN, Pfeiffenberger E o.a. AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nature Communications. 2020;11(1). 1032. https://doi.org/10.1038/s41467-020-14812-0

Author

Dohmen, Marc ; Krieg, Sarah ; Agalaridis, Georgios ; Zhu, Xiaoqing ; Shehata, Saifeldin N ; Pfeiffenberger, Elisabeth ; Amelang, Jan ; Bütepage, Mareike ; Buerova, Elena ; Pfaff, Carolina M ; Chanda, Dipanjan ; Geley, Stephan ; Preisinger, Christian ; Sakamoto, Kei ; Lüscher, Bernhard ; Neumann, Dietbert ; Vervoorts, Jörg. / AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. I: Nature Communications. 2020 ; Bind 11, Nr. 1.

Bibtex

@article{e66c282209aa4fe5b5d3bd63f9527f3f,
title = "AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy",
abstract = "The AMP-activated protein kinase (AMPK) is a master sensor of the cellular energy status that is crucial for the adaptive response to limited energy availability. AMPK is implicated in the regulation of many cellular processes, including autophagy. However, the precise mechanisms by which AMPK controls these processes and the identities of relevant substrates are not fully understood. Using protein microarrays, we identify Cyclin Y as an AMPK substrate that is phosphorylated at Serine 326 (S326) both in vitro and in cells. Phosphorylation of Cyclin Y at S326 promotes its interaction with the Cyclin-dependent kinase 16 (CDK16), thereby stimulating its catalytic activity. When expressed in cells, Cyclin Y/CDK16 is sufficient to promote autophagy. Moreover, Cyclin Y/CDK16 is necessary for efficient AMPK-dependent activation of autophagy. This functional interaction is mediated by AMPK phosphorylating S326 of Cyclin Y. Collectively, we define Cyclin Y/CDK16 as downstream effector of AMPK for inducing autophagy.",
author = "Marc Dohmen and Sarah Krieg and Georgios Agalaridis and Xiaoqing Zhu and Shehata, {Saifeldin N} and Elisabeth Pfeiffenberger and Jan Amelang and Mareike B{\"u}tepage and Elena Buerova and Pfaff, {Carolina M} and Dipanjan Chanda and Stephan Geley and Christian Preisinger and Kei Sakamoto and Bernhard L{\"u}scher and Dietbert Neumann and J{\"o}rg Vervoorts",
year = "2020",
doi = "10.1038/s41467-020-14812-0",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy

AU - Dohmen, Marc

AU - Krieg, Sarah

AU - Agalaridis, Georgios

AU - Zhu, Xiaoqing

AU - Shehata, Saifeldin N

AU - Pfeiffenberger, Elisabeth

AU - Amelang, Jan

AU - Bütepage, Mareike

AU - Buerova, Elena

AU - Pfaff, Carolina M

AU - Chanda, Dipanjan

AU - Geley, Stephan

AU - Preisinger, Christian

AU - Sakamoto, Kei

AU - Lüscher, Bernhard

AU - Neumann, Dietbert

AU - Vervoorts, Jörg

PY - 2020

Y1 - 2020

N2 - The AMP-activated protein kinase (AMPK) is a master sensor of the cellular energy status that is crucial for the adaptive response to limited energy availability. AMPK is implicated in the regulation of many cellular processes, including autophagy. However, the precise mechanisms by which AMPK controls these processes and the identities of relevant substrates are not fully understood. Using protein microarrays, we identify Cyclin Y as an AMPK substrate that is phosphorylated at Serine 326 (S326) both in vitro and in cells. Phosphorylation of Cyclin Y at S326 promotes its interaction with the Cyclin-dependent kinase 16 (CDK16), thereby stimulating its catalytic activity. When expressed in cells, Cyclin Y/CDK16 is sufficient to promote autophagy. Moreover, Cyclin Y/CDK16 is necessary for efficient AMPK-dependent activation of autophagy. This functional interaction is mediated by AMPK phosphorylating S326 of Cyclin Y. Collectively, we define Cyclin Y/CDK16 as downstream effector of AMPK for inducing autophagy.

AB - The AMP-activated protein kinase (AMPK) is a master sensor of the cellular energy status that is crucial for the adaptive response to limited energy availability. AMPK is implicated in the regulation of many cellular processes, including autophagy. However, the precise mechanisms by which AMPK controls these processes and the identities of relevant substrates are not fully understood. Using protein microarrays, we identify Cyclin Y as an AMPK substrate that is phosphorylated at Serine 326 (S326) both in vitro and in cells. Phosphorylation of Cyclin Y at S326 promotes its interaction with the Cyclin-dependent kinase 16 (CDK16), thereby stimulating its catalytic activity. When expressed in cells, Cyclin Y/CDK16 is sufficient to promote autophagy. Moreover, Cyclin Y/CDK16 is necessary for efficient AMPK-dependent activation of autophagy. This functional interaction is mediated by AMPK phosphorylating S326 of Cyclin Y. Collectively, we define Cyclin Y/CDK16 as downstream effector of AMPK for inducing autophagy.

U2 - 10.1038/s41467-020-14812-0

DO - 10.1038/s41467-020-14812-0

M3 - Journal article

C2 - 32098961

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1032

ER -

ID: 239920775