Amphipols from A to Z
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Amphipols from A to Z. / Popot, J-L; Althoff, T; Bagnard, D; Banères, J-L; Bazzacco, P; Billon-Denis, E; Catoire, Laurent J. ; Champeil, P; Charvolin, D; Cocco, M J; Crémel, G; Dahmane, T; de la Maza, L M; Ebel, C; Gabel, F; Giusti, F; Gohon, Y; Goormaghtigh, E; Guittet, E; Kleinschmidt, J. H. ; Kühlbrandt, W; Le Bon, C; Martinez, Karen Laurence; Picard, M; Pucci, B; Sachs, J N ; Tribet, C; Van Heijenoort, C; Wien, F; Zito, F; Zoonens, M.
I: Annual Review of Biophysics, Bind 40, 06.2011, s. 379-408.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Amphipols from A to Z
AU - Popot, J-L
AU - Althoff, T
AU - Bagnard, D
AU - Banères, J-L
AU - Bazzacco, P
AU - Billon-Denis, E
AU - Catoire, Laurent J.
AU - Champeil, P
AU - Charvolin, D
AU - Cocco, M J
AU - Crémel, G
AU - Dahmane, T
AU - de la Maza, L M
AU - Ebel, C
AU - Gabel, F
AU - Giusti, F
AU - Gohon, Y
AU - Goormaghtigh, E
AU - Guittet, E
AU - Kleinschmidt, J. H.
AU - Kühlbrandt, W
AU - Le Bon, C
AU - Martinez, Karen Laurence
AU - Picard, M
AU - Pucci, B
AU - Sachs, J N
AU - Tribet, C
AU - Van Heijenoort, C
AU - Wien, F
AU - Zito, F
AU - Zoonens, M
PY - 2011/6
Y1 - 2011/6
N2 - Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.
AB - Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.
U2 - 10.1146/annurev-biophys-042910-155219
DO - 10.1146/annurev-biophys-042910-155219
M3 - Journal article
C2 - 21545287
VL - 40
SP - 379
EP - 408
JO - Annual Review of Biophysics
JF - Annual Review of Biophysics
SN - 1936-122X
ER -
ID: 33826425