A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. / Narimatsu, Yoshiki; Joshi, Hiren J; Zhang, Yang; Gomes, Catarina; Chen, Yen-Hsi; Lorenzetti, Flaminia; Furukawa, Sanae; Schjoldager, Katrine; Hansen, Lars; Clausen, Henrik; Bennett, Eric P; Wandall, Hans H.

I: Glycobiology, Bind 28, Nr. 5, 2018, s. 295–305.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Narimatsu, Y, Joshi, HJ, Zhang, Y, Gomes, C, Chen, Y-H, Lorenzetti, F, Furukawa, S, Schjoldager, K, Hansen, L, Clausen, H, Bennett, EP & Wandall, HH 2018, 'A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome', Glycobiology, bind 28, nr. 5, s. 295–305. https://doi.org/10.1093/glycob/cwx101

APA

Narimatsu, Y., Joshi, H. J., Zhang, Y., Gomes, C., Chen, Y-H., Lorenzetti, F., Furukawa, S., Schjoldager, K., Hansen, L., Clausen, H., Bennett, E. P., & Wandall, H. H. (2018). A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. Glycobiology, 28(5), 295–305. https://doi.org/10.1093/glycob/cwx101

Vancouver

Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen Y-H, Lorenzetti F o.a. A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. Glycobiology. 2018;28(5):295–305. https://doi.org/10.1093/glycob/cwx101

Author

Narimatsu, Yoshiki ; Joshi, Hiren J ; Zhang, Yang ; Gomes, Catarina ; Chen, Yen-Hsi ; Lorenzetti, Flaminia ; Furukawa, Sanae ; Schjoldager, Katrine ; Hansen, Lars ; Clausen, Henrik ; Bennett, Eric P ; Wandall, Hans H. / A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. I: Glycobiology. 2018 ; Bind 28, Nr. 5. s. 295–305.

Bibtex

@article{19d586295ba24ff2b17234188f48b8f5,
title = "A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome",
abstract = "Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome , which is comprised of all glycan structures found on different glycoconjugates in cells. The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins. In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence. Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis. However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures. This is especially true for glycosylation steps covered by isoenzyme gene families. Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.",
author = "Yoshiki Narimatsu and Joshi, {Hiren J} and Yang Zhang and Catarina Gomes and Yen-Hsi Chen and Flaminia Lorenzetti and Sanae Furukawa and Katrine Schjoldager and Lars Hansen and Henrik Clausen and Bennett, {Eric P} and Wandall, {Hans H}",
note = "{\textcopyright} The Author(s) 2018. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.",
year = "2018",
doi = "10.1093/glycob/cwx101",
language = "English",
volume = "28",
pages = "295–305",
journal = "Glycobiology",
issn = "0959-6658",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome

AU - Narimatsu, Yoshiki

AU - Joshi, Hiren J

AU - Zhang, Yang

AU - Gomes, Catarina

AU - Chen, Yen-Hsi

AU - Lorenzetti, Flaminia

AU - Furukawa, Sanae

AU - Schjoldager, Katrine

AU - Hansen, Lars

AU - Clausen, Henrik

AU - Bennett, Eric P

AU - Wandall, Hans H

N1 - © The Author(s) 2018. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2018

Y1 - 2018

N2 - Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome , which is comprised of all glycan structures found on different glycoconjugates in cells. The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins. In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence. Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis. However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures. This is especially true for glycosylation steps covered by isoenzyme gene families. Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.

AB - Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome , which is comprised of all glycan structures found on different glycoconjugates in cells. The glycome is vast, and despite advancements in analytic strategies it continues to be difficult to decipher biological roles of glycans with respect to specific glycan structures, type of glycoconjugate, particular glycoproteins, and distinct glycosites on proteins. In contrast to this, the number of glycosyltransferase genes involved in the biosynthesis of the human glycome is manageable, and the biosynthetic roles of most of these enzymes are defined or can be predicted with reasonable confidence. Thus, with the availability of the facile CRISPR/Cas9 gene editing tool it now seems easier to approach investigation of the functions of the glycome through genetic dissection of biosynthetic pathways, rather than by direct glycan analysis. However, obstacles still remain with design and validation of efficient gene targeting constructs, as well as with the interpretation of results from gene targeting and the translation of gene function to glycan structures. This is especially true for glycosylation steps covered by isoenzyme gene families. Here, we present a library of validated high-efficiency gRNA designs suitable for individual and combinatorial targeting of the human glycosyltransferase genome together with a global view of the predicted functions of human glycosyltransferases to facilitate and guide gene targeting strategies in studies of the human glycome.

U2 - 10.1093/glycob/cwx101

DO - 10.1093/glycob/cwx101

M3 - Journal article

C2 - 29315387

VL - 28

SP - 295

EP - 305

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 5

ER -

ID: 196435495