TY - JOUR

T1 - A single bout of one-legged exercise to local exhaustion decreases insulin action in non-exercised muscle leading to decreased whole-body insulin action

AU - Steenberg, Dorte Enggaard

AU - Hingst, Janne Rasmuss

AU - Birk, Jesper Bratz

AU - Thorup, Anette

AU - Kristensen, Jonas Møller

AU - Sjøberg, Kim Anker

AU - Kiens, Bente

AU - Richter, Erik A.

AU - Wojtaszewski, Jørgen

N1 - © 2020 by the American Diabetes Association.

PY - 2020

Y1 - 2020

N2 - A single bout of exercise enhances insulin action in the exercised muscle. However, not all human studies find that this translates into increased whole-body insulin action, suggesting that insulin action in rested muscle or other organs may be decreased by exercise. To investigate this, eight healthy men underwent a euglycemic hyperinsulinemic clamp on two separate days: One day with prior one-legged knee-extensor exercise to local exhaustion (∼2.5 hours) and another day without exercise. Whole-body glucose disposal was ∼18% lower on the exercise day as compared to the resting day due to decreased (-37%) insulin-stimulated glucose uptake in the non-exercised muscle. Insulin signaling at the level of Akt2 was impaired in the non-exercised muscle on the exercise day suggesting that decreased insulin action in non-exercised muscle may reduce GLUT4 translocation in response to insulin.Thus, the effect of a single bout of exercise on whole-body insulin action depends on the balance between local effects increasing and systemic effects decreasing insulin action. Physiologically, this mechanism may serve to direct glucose into the muscles in need of glycogen replenishment. For insulin-treated patients this complex relationship may explain the difficulties in predicting the adequate insulin dose for maintaining glucose homeostasis following physical activity.

AB - A single bout of exercise enhances insulin action in the exercised muscle. However, not all human studies find that this translates into increased whole-body insulin action, suggesting that insulin action in rested muscle or other organs may be decreased by exercise. To investigate this, eight healthy men underwent a euglycemic hyperinsulinemic clamp on two separate days: One day with prior one-legged knee-extensor exercise to local exhaustion (∼2.5 hours) and another day without exercise. Whole-body glucose disposal was ∼18% lower on the exercise day as compared to the resting day due to decreased (-37%) insulin-stimulated glucose uptake in the non-exercised muscle. Insulin signaling at the level of Akt2 was impaired in the non-exercised muscle on the exercise day suggesting that decreased insulin action in non-exercised muscle may reduce GLUT4 translocation in response to insulin.Thus, the effect of a single bout of exercise on whole-body insulin action depends on the balance between local effects increasing and systemic effects decreasing insulin action. Physiologically, this mechanism may serve to direct glucose into the muscles in need of glycogen replenishment. For insulin-treated patients this complex relationship may explain the difficulties in predicting the adequate insulin dose for maintaining glucose homeostasis following physical activity.

KW - Faculty of Science

KW - Insulin sensitivity

KW - Insulin resistance

KW - Glucose uptake

U2 - 10.2337/db19-1010

DO - 10.2337/db19-1010

M3 - Journal article

C2 - 31974138

VL - 69

SP - 578

EP - 590

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 4

ER -