A simple method for the parallel deep sequencing of full influenza A genomes

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Standard

A simple method for the parallel deep sequencing of full influenza A genomes. / Kampmann, Marie-Louise; Fordyce, Sarah Louise; Avila Arcos, Maria del Carmen; Rasmussen, Morten; Willerslev, Eske; Nielsen, Lars Peter; Gilbert, Tom.

I: Journal of Virological Methods, Bind 178, Nr. 1-2, 12.2011, s. 243-248.

Publikation: Bidrag til tidsskrift › Letter › Forskning › fagfællebedømt

Harvard

Kampmann, M-L, Fordyce, SL, Avila Arcos, MDC, Rasmussen, M, Willerslev, E, Nielsen, LP & Gilbert, T 2011, 'A simple method for the parallel deep sequencing of full influenza A genomes', Journal of Virological Methods, bind 178, nr. 1-2, s. 243-248. https://doi.org/10.1016/j.jviromet.2011.09.001

APA

Kampmann, M-L., Fordyce, S. L., Avila Arcos, M. D. C., Rasmussen, M., Willerslev, E., Nielsen, L. P., & Gilbert, T. (2011). A simple method for the parallel deep sequencing of full influenza A genomes. Journal of Virological Methods, 178(1-2), 243-248. https://doi.org/10.1016/j.jviromet.2011.09.001

Vancouver

Kampmann M-L, Fordyce SL, Avila Arcos MDC, Rasmussen M, Willerslev E, Nielsen LP o.a. A simple method for the parallel deep sequencing of full influenza A genomes. Journal of Virological Methods. 2011 dec.;178(1-2):243-248. https://doi.org/10.1016/j.jviromet.2011.09.001

Author

Kampmann, Marie-Louise ; Fordyce, Sarah Louise ; Avila Arcos, Maria del Carmen ; Rasmussen, Morten ; Willerslev, Eske ; Nielsen, Lars Peter ; Gilbert, Tom. / A simple method for the parallel deep sequencing of full influenza A genomes. I: Journal of Virological Methods. 2011 ; Bind 178, Nr. 1-2. s. 243-248.

Bibtex

@article{d3da5d11ca604b33acaa854d7a76ea53,
title = "A simple method for the parallel deep sequencing of full influenza A genomes",
abstract = "Given the major threat of influenza A to human and animal health, and its ability to evolve rapidly through mutation and reassortment, tools that enable its timely characterization are necessary to help monitor its evolution and spread. For this purpose, deep sequencing can be a very valuable tool. This study reports a comprehensive method that enables deep sequencing of the complete genomes of influenza A subtypes using the Illumina Genome Analyzer IIx (GAIIx). By using this method, the complete genomes of nine viruses were sequenced in parallel, representing the 2009 pandemic H1N1 virus, H5N1 virus from human and H1N1 virus from swine, on a single lane of a GAIIx flow cell to an average depth of 122-fold. This technique can be applied to cultivated and uncultivated virus.",
keywords = "Faculty of Science, Influenza A , Full genome sequencing, High-throughput sequencing ",
author = "Marie-Louise Kampmann and Fordyce, {Sarah Louise} and {Avila Arcos}, {Maria del Carmen} and Morten Rasmussen and Eske Willerslev and Nielsen, {Lars Peter} and Tom Gilbert",
year = "2011",
month = dec,
doi = "10.1016/j.jviromet.2011.09.001",
language = "English",
volume = "178",
pages = "243--248",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - A simple method for the parallel deep sequencing of full influenza A genomes

AU - Kampmann, Marie-Louise

AU - Fordyce, Sarah Louise

AU - Avila Arcos, Maria del Carmen

AU - Rasmussen, Morten

AU - Willerslev, Eske

AU - Nielsen, Lars Peter

AU - Gilbert, Tom

PY - 2011/12

Y1 - 2011/12

N2 - Given the major threat of influenza A to human and animal health, and its ability to evolve rapidly through mutation and reassortment, tools that enable its timely characterization are necessary to help monitor its evolution and spread. For this purpose, deep sequencing can be a very valuable tool. This study reports a comprehensive method that enables deep sequencing of the complete genomes of influenza A subtypes using the Illumina Genome Analyzer IIx (GAIIx). By using this method, the complete genomes of nine viruses were sequenced in parallel, representing the 2009 pandemic H1N1 virus, H5N1 virus from human and H1N1 virus from swine, on a single lane of a GAIIx flow cell to an average depth of 122-fold. This technique can be applied to cultivated and uncultivated virus.

AB - Given the major threat of influenza A to human and animal health, and its ability to evolve rapidly through mutation and reassortment, tools that enable its timely characterization are necessary to help monitor its evolution and spread. For this purpose, deep sequencing can be a very valuable tool. This study reports a comprehensive method that enables deep sequencing of the complete genomes of influenza A subtypes using the Illumina Genome Analyzer IIx (GAIIx). By using this method, the complete genomes of nine viruses were sequenced in parallel, representing the 2009 pandemic H1N1 virus, H5N1 virus from human and H1N1 virus from swine, on a single lane of a GAIIx flow cell to an average depth of 122-fold. This technique can be applied to cultivated and uncultivated virus.

KW - Faculty of Science

KW - Influenza A

KW - Full genome sequencing

KW - High-throughput sequencing

U2 - 10.1016/j.jviromet.2011.09.001

DO - 10.1016/j.jviromet.2011.09.001

M3 - Letter

VL - 178

SP - 243

EP - 248

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

IS - 1-2

ER -

ID: 35956274