A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence

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Standard

A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence. / Kooistra, Susanne Marije; Rudkjær, Lise Christine; Lees, Michael James; Steinhauer, Cornelia; Johansen, Jens Vilstrup; Helin, Kristian.

I: PLOS ONE, Bind 9, Nr. 3, e91034, 2014, s. 1-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kooistra, SM, Rudkjær, LC, Lees, MJ, Steinhauer, C, Johansen, JV & Helin, K 2014, 'A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence', PLOS ONE, bind 9, nr. 3, e91034, s. 1-7. https://doi.org/10.1371/journal.pone.0091034

APA

Kooistra, S. M., Rudkjær, L. C., Lees, M. J., Steinhauer, C., Johansen, J. V., & Helin, K. (2014). A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence. PLOS ONE, 9(3), 1-7. [e91034]. https://doi.org/10.1371/journal.pone.0091034

Vancouver

Kooistra SM, Rudkjær LC, Lees MJ, Steinhauer C, Johansen JV, Helin K. A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence. PLOS ONE. 2014;9(3):1-7. e91034. https://doi.org/10.1371/journal.pone.0091034

Author

Kooistra, Susanne Marije ; Rudkjær, Lise Christine ; Lees, Michael James ; Steinhauer, Cornelia ; Johansen, Jens Vilstrup ; Helin, Kristian. / A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence. I: PLOS ONE. 2014 ; Bind 9, Nr. 3. s. 1-7.

Bibtex

@article{3c4b74d6b2c8475daffd363ec00dbf05,
title = "A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence",
abstract = "Oncogene-induced senescence (OIS) can occur in response to hyperactive oncogenic signals and is believed to be a fail-safe mechanism protecting against tumorigenesis. To identify new factors involved in OIS, we performed a screen for microRNAs that can overcome or inhibit OIS in human diploid fibroblasts. This screen led to the identification of miR-378a-5p and in addition several other miRNAs that have previously been shown to play a role in senescence. We show that ectopic expression of miR-378a-5p reduces the expression of several senescence markers, including p16INK4A and senescence-associated β-galactosidase. Moreover, cells with ectopic expression of miR-378a-5p retain proliferative capacity even in the presence of an activated Braf oncogene. Finally, we identified several miR-378a-5p targets in diploid fibroblasts that might explain the mechanism by which the microRNA can delay OIS. We speculate that miR-378a-5p might positively influence tumor formation by delaying OIS, which is consistent with a known pro-oncogenic function of this microRNA.",
author = "Kooistra, {Susanne Marije} and Rudkj{\ae}r, {Lise Christine} and Lees, {Michael James} and Cornelia Steinhauer and Johansen, {Jens Vilstrup} and Kristian Helin",
year = "2014",
doi = "10.1371/journal.pone.0091034",
language = "English",
volume = "9",
pages = "1--7",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - A Screen Identifies the Oncogenic Micro-RNA miR-378a-5p as a Negative Regulator of Oncogene-Induced Senescence

AU - Kooistra, Susanne Marije

AU - Rudkjær, Lise Christine

AU - Lees, Michael James

AU - Steinhauer, Cornelia

AU - Johansen, Jens Vilstrup

AU - Helin, Kristian

PY - 2014

Y1 - 2014

N2 - Oncogene-induced senescence (OIS) can occur in response to hyperactive oncogenic signals and is believed to be a fail-safe mechanism protecting against tumorigenesis. To identify new factors involved in OIS, we performed a screen for microRNAs that can overcome or inhibit OIS in human diploid fibroblasts. This screen led to the identification of miR-378a-5p and in addition several other miRNAs that have previously been shown to play a role in senescence. We show that ectopic expression of miR-378a-5p reduces the expression of several senescence markers, including p16INK4A and senescence-associated β-galactosidase. Moreover, cells with ectopic expression of miR-378a-5p retain proliferative capacity even in the presence of an activated Braf oncogene. Finally, we identified several miR-378a-5p targets in diploid fibroblasts that might explain the mechanism by which the microRNA can delay OIS. We speculate that miR-378a-5p might positively influence tumor formation by delaying OIS, which is consistent with a known pro-oncogenic function of this microRNA.

AB - Oncogene-induced senescence (OIS) can occur in response to hyperactive oncogenic signals and is believed to be a fail-safe mechanism protecting against tumorigenesis. To identify new factors involved in OIS, we performed a screen for microRNAs that can overcome or inhibit OIS in human diploid fibroblasts. This screen led to the identification of miR-378a-5p and in addition several other miRNAs that have previously been shown to play a role in senescence. We show that ectopic expression of miR-378a-5p reduces the expression of several senescence markers, including p16INK4A and senescence-associated β-galactosidase. Moreover, cells with ectopic expression of miR-378a-5p retain proliferative capacity even in the presence of an activated Braf oncogene. Finally, we identified several miR-378a-5p targets in diploid fibroblasts that might explain the mechanism by which the microRNA can delay OIS. We speculate that miR-378a-5p might positively influence tumor formation by delaying OIS, which is consistent with a known pro-oncogenic function of this microRNA.

U2 - 10.1371/journal.pone.0091034

DO - 10.1371/journal.pone.0091034

M3 - Journal article

C2 - 24651706

VL - 9

SP - 1

EP - 7

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 3

M1 - e91034

ER -

ID: 105328749