Alpha-adrenergic receptors in rat skeletal muscle
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Alpha-adrenergic receptors in rat skeletal muscle. / Rattigan, S; Appleby, G J; Edwards, S J; McKinstry, W J; Colquhoun, E Q; Clark, M G; Richter, Erik A.
I: Biochemical and Biophysical Research Communications, Bind 136, Nr. 3, 1986, s. 1071-1077.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Alpha-adrenergic receptors in rat skeletal muscle
AU - Rattigan, S
AU - Appleby, G J
AU - Edwards, S J
AU - McKinstry, W J
AU - Colquhoun, E Q
AU - Clark, M G
AU - Richter, Erik A.
PY - 1986
Y1 - 1986
N2 - Sarcolemma-enriched preparations from muscles rich in slow oxidative red fibres contained specific binding sites for the alpha 1 antagonist, prazosin (e.g. soleus Kd 0.13 nM, Bmax 29 fmol/mg protein). Binding sites for prazosin were almost absent from white muscle. Displacement of prazosin binding from sarcolemma of soleus muscle (phentolamine greater than phenylephrine greater than idazoxan greater than yohimbine) suggested that the receptors were alpha 1. Binding sites for dihydroalprenolol (beta antagonist) were also more concentrated on red than white muscle and outnumbered prazosin sites by approx. 10:1. Binding sites for idazoxan (alpha 2 antagonist) were undetectable. Contamination of sarcolemma-enriched preparations by endothelial tissue indicated by the activity of angiotensin converting enzyme did not correlate with prazosin binding. It is concluded that post-synaptic alpha 1 adrenergic receptors are present on the sarcolemma of slow oxidative red fibres of rat skeletal muscle. The presence provides the mechanistic basis for apparent alpha-adrenergic effects to increase glucose and oxygen uptake in perfused rat hindquarter.
AB - Sarcolemma-enriched preparations from muscles rich in slow oxidative red fibres contained specific binding sites for the alpha 1 antagonist, prazosin (e.g. soleus Kd 0.13 nM, Bmax 29 fmol/mg protein). Binding sites for prazosin were almost absent from white muscle. Displacement of prazosin binding from sarcolemma of soleus muscle (phentolamine greater than phenylephrine greater than idazoxan greater than yohimbine) suggested that the receptors were alpha 1. Binding sites for dihydroalprenolol (beta antagonist) were also more concentrated on red than white muscle and outnumbered prazosin sites by approx. 10:1. Binding sites for idazoxan (alpha 2 antagonist) were undetectable. Contamination of sarcolemma-enriched preparations by endothelial tissue indicated by the activity of angiotensin converting enzyme did not correlate with prazosin binding. It is concluded that post-synaptic alpha 1 adrenergic receptors are present on the sarcolemma of slow oxidative red fibres of rat skeletal muscle. The presence provides the mechanistic basis for apparent alpha-adrenergic effects to increase glucose and oxygen uptake in perfused rat hindquarter.
KW - Animals
KW - Binding Sites
KW - Dihydroalprenolol
KW - Dioxanes
KW - Female
KW - Idazoxan
KW - Kinetics
KW - Male
KW - Muscles
KW - Peptidyl-Dipeptidase A
KW - Phentolamine
KW - Phenylephrine
KW - Prazosin
KW - Rats
KW - Receptors, Adrenergic, alpha
KW - Sarcolemma
KW - Yohimbine
M3 - Journal article
C2 - 3013164
VL - 136
SP - 1071
EP - 1077
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -
ID: 154758373