Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent

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Jacob Jeppesen, Peter Hjorth Albers, Adam John Rose, Jesper Bratz Birk, Peter Schjerling, Nicolas Dzamko, Gregory R. Steinberg, Bente Kiens

The aim of this study was to investigate the molecular mechanisms regulating FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. In one model, WT and AMPK KD mice were exercised or EDL and SOL muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and fatty acid uptake in response to muscle contractions was investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, AICAR only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions associated with increased fatty acid uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.
OriginalsprogEngelsk
TidsskriftJournal of Lipid Research
Vol/bind52
Udgave nummer4
Sider (fra-til)699-711
Antal sider13
ISSN0022-2275
DOI
StatusUdgivet - 2011

Bibliografisk note

CURIS 2011 5200 026

ID: 32928268