Development of a multiplex PCR assay detecting 52 autosomal SNPs

Publikation: Bidrag til bog/antologi/rapportKonferencebidrag i proceedingsForskningfagfællebedømt

Juan Jose Sanchez Sanchez, C. Phillips, Claus Børsting, M. Bogus, A. Carracedo, Denise Syndercombe-Court, M. Fondevila, C.D. Harrison, N. Morling, K. Balogh, Peter M. Schneider, SNPforID Consortium

An efficient method that can be used to simultaneously amplify a set of genetic loci across the genome with high reliability can provide a valuable tool for single nucleotide polymorphism (SNP) forensic genotyping. A crucial element is the number of individual biochemical reactions that must be performed. The SNPforID consortium (www.snpforid.org) was established in 2003 with the principal goal of developing a SNP-based system of DNA analysis that would have comparable discrimination power and ease of use to those of existing short tandem repeat (STR) based techniques. Here, we describe a strategy for amplifying 52 genomic DNA fragments, each containing one SNP, in a single tube, and accurately genotyping the PCR product mixture using two single base extension reactions. This multiplex approach reduces the cost of SNP genotyping and requires as little as 0.5 ng of genomic DNA to detect 52 SNPs. We used a multiple injection approach for DNA sequencers that can effectively detect all the SNPs amplified in a single electrophoretic run. We present SNP data for 700 unrelated individuals from 9 populations
OriginalsprogEngelsk
TitelProgress in Forensic Genetics 11 : Proceedings of the 21st International ISFG Congress
Antal sider2
Vol/bind1288
ForlagElsevier
Publikationsdato2006
Sider67-69
StatusUdgivet - 2006
Begivenhed21st International ISFG Congress - Ponta Delgada, The Azores, Portugal
Varighed: 13 sep. 200516 sep. 2005

Konference

Konference21st International ISFG Congress
LandPortugal
ByPonta Delgada, The Azores
Periode13/09/200516/09/2005
NavnICS - International Congress Series
Vol/bind1288

ID: 18055053