Immune monitoring using mRNA-transfected dendritic cells

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Troels Holz Borch, Inge Marie Svane, Özcan Met

Dendritic cells are known to be the most potent antigen presenting cell in the immune system and are used as cellular adjuvants in therapeutic anticancer vaccines using various tumor-associated antigens or their derivatives. One way of loading antigen into the dendritic cells is by mRNA electroporation, ensuring presentation of antigen through major histocompatibility complex I and potentially activating T cells, enabling them to kill the tumor cells. Despite extensive research in the field, only one dendritic cell-based vaccine has been approved. There is therefore a great need to elucidate and understand the immunological impact of dendritic cell vaccination in order to improve clinical benefit. In this chapter, we describe a method for performing immune monitoring using peripheral blood mononuclear cells and autologous dendritic cells transfected with tumor-associated antigen-encoding mRNA.

TitelSynthetic mRNA : Production, Introduction Into Cells, and Physiological Consequences
Antal sider15
ForlagHumana Press
ISBN (Trykt)978-1-4939-3623-6
ISBN (Elektronisk)978-1-4939-3625-0
StatusUdgivet - 2016
NavnMethods in Molecular Biology

ID: 176375911