Uranyl Photocleavage of Phosphopeptides Yields Truncated C-Terminally Amidated Peptide Products

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Rasmus L B Elnegaard, Niels Erik Møllegaard, Qiang Zhang, Frank Kjeldsen, Thomas J D Jørgensen

The uranyl ion (UO2(2+) ) binds phosphopeptides with high affinity, and when irradiated with UV-light, it can cleave the peptide backbone. In this study, high-accuracy tandem mass spectrometry and enzymatic assays were used to characterise the photocleavage products resulting from the uranyl photocleavage reaction of a tetraphosphorylated β-casein model peptide. We show that the primary photocleavage products of the uranyl-catalysed reaction are C-terminally amidated. This could be of great interest to the pharmaceutical industry, as efficient peptide amidation reactions are one of the top challenges in green pharmaceutical chemistry.

OriginalsprogEngelsk
TidsskriftChemBioChem
Vol/bind18
Udgave nummer12
Sider (fra-til)1117-1122
Antal sider6
ISSN1439-4227
DOI
StatusUdgivet - 19 jun. 2017

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