The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence

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The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence. / Bartelt, Stine; Timm, Michael; Damsgaard, Camilla Trab; Hansen, Erik Wind; Hansen, Harald S.; Lauritzen, Lotte.

I: British Journal of Nutrition, Bind 99, Nr. 6, 2008, s. 1230-1238.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bartelt, S, Timm, M, Damsgaard, CT, Hansen, EW, Hansen, HS & Lauritzen, L 2008, 'The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence', British Journal of Nutrition, bind 99, nr. 6, s. 1230-1238. https://doi.org/10.1017/S0007114507853451

APA

Bartelt, S., Timm, M., Damsgaard, C. T., Hansen, E. W., Hansen, H. S., & Lauritzen, L. (2008). The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence. British Journal of Nutrition, 99(6), 1230-1238. https://doi.org/10.1017/S0007114507853451

Vancouver

Bartelt S, Timm M, Damsgaard CT, Hansen EW, Hansen HS, Lauritzen L. The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence. British Journal of Nutrition. 2008;99(6):1230-1238. https://doi.org/10.1017/S0007114507853451

Author

Bartelt, Stine ; Timm, Michael ; Damsgaard, Camilla Trab ; Hansen, Erik Wind ; Hansen, Harald S. ; Lauritzen, Lotte. / The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence. I: British Journal of Nutrition. 2008 ; Bind 99, Nr. 6. s. 1230-1238.

Bibtex

@article{a6b7c920a1c311ddb6ae000ea68e967b,
title = "The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence",
abstract = "Dietary long-chain n-3 PUFA (n-3 LCPUFA) are thought to have immune-modulating effects, but the specific effects and mechanisms are not fully elucidated. The aim of this study was to determine whether dietary n-3 LCPUFA could affect ex vivo oxidative burst in healthy young men. The study had a randomised 2 £ 2-factorial design in which subjects were randomly assigned to 8-week supplementation with capsules containing fish oil (about 29 g n-3 LCPUFA/d) or olive oil (control). Subjects were also randomly assigned to household use of oils and fat spreads with a high or a low 18 : 2n-6 content. At baseline and at the end of the intervention, the fatty acid composition of peripheral blood mononuclear cells (PBMC) was analysed by GLC and oxidative burst was studied in whole blood stimulated with zymosan using luminol-enhanced chemiluminescence. The PBMC content of n-3 LCPUFA was markedly increased by the fish oil-supplementation (P,0001, compared to the olive oil groups). No effect of the intervention was observed on neutrophil count, but one measure of the zymosan-induced oxidative burst was higher in the fish oil groups (P¼003) compared to the olive oil groups. The fat intervention did not in itself affect oxidative burst neither did it change the effect of the fishoil intervention. The measures of oxidative burst at the end of the intervention period were found to be associated with the DHA content of PBMC (r 044, P¼0016), suggesting a dose-response relationship. These results indicate that n-3 LCPUFA may have immuno-stimulating effects.",
author = "Stine Bartelt and Michael Timm and Damsgaard, {Camilla Trab} and Hansen, {Erik Wind} and Hansen, {Harald S.} and Lotte Lauritzen",
year = "2008",
doi = "10.1017/S0007114507853451",
language = "English",
volume = "99",
pages = "1230--1238",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "6",

}

RIS

TY - JOUR

T1 - The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence

AU - Bartelt, Stine

AU - Timm, Michael

AU - Damsgaard, Camilla Trab

AU - Hansen, Erik Wind

AU - Hansen, Harald S.

AU - Lauritzen, Lotte

PY - 2008

Y1 - 2008

N2 - Dietary long-chain n-3 PUFA (n-3 LCPUFA) are thought to have immune-modulating effects, but the specific effects and mechanisms are not fully elucidated. The aim of this study was to determine whether dietary n-3 LCPUFA could affect ex vivo oxidative burst in healthy young men. The study had a randomised 2 £ 2-factorial design in which subjects were randomly assigned to 8-week supplementation with capsules containing fish oil (about 29 g n-3 LCPUFA/d) or olive oil (control). Subjects were also randomly assigned to household use of oils and fat spreads with a high or a low 18 : 2n-6 content. At baseline and at the end of the intervention, the fatty acid composition of peripheral blood mononuclear cells (PBMC) was analysed by GLC and oxidative burst was studied in whole blood stimulated with zymosan using luminol-enhanced chemiluminescence. The PBMC content of n-3 LCPUFA was markedly increased by the fish oil-supplementation (P,0001, compared to the olive oil groups). No effect of the intervention was observed on neutrophil count, but one measure of the zymosan-induced oxidative burst was higher in the fish oil groups (P¼003) compared to the olive oil groups. The fat intervention did not in itself affect oxidative burst neither did it change the effect of the fishoil intervention. The measures of oxidative burst at the end of the intervention period were found to be associated with the DHA content of PBMC (r 044, P¼0016), suggesting a dose-response relationship. These results indicate that n-3 LCPUFA may have immuno-stimulating effects.

AB - Dietary long-chain n-3 PUFA (n-3 LCPUFA) are thought to have immune-modulating effects, but the specific effects and mechanisms are not fully elucidated. The aim of this study was to determine whether dietary n-3 LCPUFA could affect ex vivo oxidative burst in healthy young men. The study had a randomised 2 £ 2-factorial design in which subjects were randomly assigned to 8-week supplementation with capsules containing fish oil (about 29 g n-3 LCPUFA/d) or olive oil (control). Subjects were also randomly assigned to household use of oils and fat spreads with a high or a low 18 : 2n-6 content. At baseline and at the end of the intervention, the fatty acid composition of peripheral blood mononuclear cells (PBMC) was analysed by GLC and oxidative burst was studied in whole blood stimulated with zymosan using luminol-enhanced chemiluminescence. The PBMC content of n-3 LCPUFA was markedly increased by the fish oil-supplementation (P,0001, compared to the olive oil groups). No effect of the intervention was observed on neutrophil count, but one measure of the zymosan-induced oxidative burst was higher in the fish oil groups (P¼003) compared to the olive oil groups. The fat intervention did not in itself affect oxidative burst neither did it change the effect of the fishoil intervention. The measures of oxidative burst at the end of the intervention period were found to be associated with the DHA content of PBMC (r 044, P¼0016), suggesting a dose-response relationship. These results indicate that n-3 LCPUFA may have immuno-stimulating effects.

U2 - 10.1017/S0007114507853451

DO - 10.1017/S0007114507853451

M3 - Journal article

VL - 99

SP - 1230

EP - 1238

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 6

ER -

ID: 8099389