Origin and evolution of transporter substrate specificity within the NPF family

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Dokumenter

Morten Egevang Jørgensen, Deyang Xu, Christoph Crocoll, Heidi Asschenfeldt Ernst, David Ramírez, Mohammed Saddik Motawie, Carl Erik Olsen, Osman Asghar Mirza, Hussam Hassan Nour-Eldin, Barbara Ann Halkier

Despite vast diversity in metabolites and the matching substrate specificity of their transporters, little is known about how evolution of transporter substrate specificities is linked to emergence of substrates via evolution of biosynthetic pathways. Transporter specificity towards the recently evolved glucosinolates characteristic of Brassicales is shown to evolve prior to emergence of glucosinolate biosynthesis. Furthermore, we show that glucosinolate transporters belonging to the ubiquitous NRT1/PTR FAMILY (NPF) likely evolved from transporters of the ancestral cyanogenic glucosides found across more than 2500 species outside of the Brassicales. Biochemical characterization of orthologs along the phylogenetic lineage from cassava to A. thaliana, suggests that alterations in the electrogenicity of the transporters accompanied changes in substrate specificity. Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new biosynthesis pathways emerge.
OriginalsprogEngelsk
Artikelnummere19466
TidsskrifteLife
Vol/bind6
Antal sider30
ISSN2050-084X
DOI
StatusUdgivet - 2017

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