No inflammatory gene-expression response to acute exercise in human Achilles tendinopathy

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Jessica Pingel, Ulrich Fredberg, Lone Ramer Mikkelsen, Peter Schjerling, Katja Maria Heinemeier, Michael Kjær, Adrian Paul Harrison, Henning Langberg

Although histology data favour the view of a degenerative nature of tendinopathy, indirect support for inflammatory reactions to loading in affected tendons exists. The purpose of the present study was to elucidate whether inflammatory signalling responses after acute mechanical loading were more pronounced in tendinopathic versus healthy regions of human tendon and if treatment with non-steroidal anti-inflammatory medications (NSAID's) reduces this response. Twenty-seven tendinopathy patients (>6 months) were randomly assigned to a placebo (n = 14) or NSAID (Ibumetin NYCOMED GmbH Plant Oranienburg Germany (600 mg) × 3/day/1 week) group (n = 13) in a double-blinded-fashion. Tendon biopsies were taken from the painful and a healthy region of the same tendon 2 h after 1 h running. Gene-expression of several targets was analysed in the sampled Achilles tendon biopsies. The mRNA for TGF-ß, collagen-I and collagen-III were significantly higher expressed, and decorin, CTGF, IL-6 and IL-10 were significantly lower expressed in the tendinopathic versus healthy tendon area. Only IL-10 was lower in expression in experiments with NSAID administration, while all other determined parameters were unaffected by NSAID. All ultrasonographic outcomes were unchanged in response to acute exercise and not influenced by NSAID. The signalling for collagen and TGF-beta was upregulated after acute loading in tendinopathic tendon. In contrast to the hypothesis, inflammatory signalling was not exaggerated in tendinopathic tendon 2 h after acute mechanical loading.
OriginalsprogEngelsk
TidsskriftEuropean Journal of Applied Physiology
Vol/bind113
Udgave nummer8
Sider (fra-til)2101-2109
Antal sider9
ISSN1439-6319
DOI
StatusUdgivet - 2013

Bibliografisk note

CURIS 2013 NEXS 181

ID: 45258288