Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Standard

Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action. / Jana, Bimal; Baker, Kristin Renee; Guardabassi, Luca.

Antimicrobial Peptides: Methods and Protocols. Bind 1548 Humana Press, 2017. s. 181-190 (Methods in Molecular Biology, Bind 1548).

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Harvard

Jana, B, Baker, KR & Guardabassi, L 2017, Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action. i Antimicrobial Peptides: Methods and Protocols. bind 1548, Humana Press, Methods in Molecular Biology, bind 1548, s. 181-190. https://doi.org/10.1007/978-1-4939-6737-7_12

APA

Jana, B., Baker, K. R., & Guardabassi, L. (2017). Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action. I Antimicrobial Peptides: Methods and Protocols (Bind 1548, s. 181-190). Humana Press. Methods in Molecular Biology, Bind. 1548 https://doi.org/10.1007/978-1-4939-6737-7_12

Vancouver

Jana B, Baker KR, Guardabassi L. Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action. I Antimicrobial Peptides: Methods and Protocols. Bind 1548. Humana Press. 2017. s. 181-190. (Methods in Molecular Biology, Bind 1548). https://doi.org/10.1007/978-1-4939-6737-7_12

Author

Jana, Bimal ; Baker, Kristin Renee ; Guardabassi, Luca. / Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action. Antimicrobial Peptides: Methods and Protocols. Bind 1548 Humana Press, 2017. s. 181-190 (Methods in Molecular Biology, Bind 1548).

Bibtex

@inbook{cc4960d09b77475ca12819d0b50efc58,
title = "Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action",
abstract = "Antimicrobial peptides (AMPs) are viable alternatives to the currently available antimicrobials, and numerous studies have investigated their possible use as therapeutic agents for specific clinical applications. AMPs are a diverse class of antimicrobials that often act upon the bacterial cell membrane but may exhibit additional modes of action. Identification of the multiple modes of action requires a comprehensive study at subinhibitory concentrations and careful data analysis since additional modes of action can be eclipsed by AMP action on the cell membrane. Techniques that measure the biosynthesis rate of macromolecules (e.g., DNA, RNA, protein, and cell wall) and the cytoplasmic membrane proton motive force (PMF) energy can help to unravel the diverse modes of action of AMPs. Here, we present an overview of macromolecule biosynthesis rate measurement and fluorescence spectroscopy methods to identify AMP mode(s) of action. Detailed protocols designed to measure inhibition of DNA, RNA, protein, and cell wall synthesis or membrane de-energization are presented and discussed for optimal application of these two techniques as well as to enable accurate interpretation of the experimental findings.",
keywords = "Antimicrobial peptide, Fluorescence spectroscopy, Macromolecule biosynthesis, Membrane de-energization/depolarization, Mode(s) of action, Proton motive force",
author = "Bimal Jana and Baker, {Kristin Renee} and Luca Guardabassi",
year = "2017",
doi = "10.1007/978-1-4939-6737-7_12",
language = "English",
isbn = "978-1-4939-6735-3",
volume = "1548",
pages = "181--190",
booktitle = "Antimicrobial Peptides",
publisher = "Humana Press",
address = "United States",

}

RIS

TY - CHAP

T1 - Macromolecule biosynthesis assay and fluorescence spectroscopy methods to explore antimicrobial peptide mode(s) of action

AU - Jana, Bimal

AU - Baker, Kristin Renee

AU - Guardabassi, Luca

PY - 2017

Y1 - 2017

N2 - Antimicrobial peptides (AMPs) are viable alternatives to the currently available antimicrobials, and numerous studies have investigated their possible use as therapeutic agents for specific clinical applications. AMPs are a diverse class of antimicrobials that often act upon the bacterial cell membrane but may exhibit additional modes of action. Identification of the multiple modes of action requires a comprehensive study at subinhibitory concentrations and careful data analysis since additional modes of action can be eclipsed by AMP action on the cell membrane. Techniques that measure the biosynthesis rate of macromolecules (e.g., DNA, RNA, protein, and cell wall) and the cytoplasmic membrane proton motive force (PMF) energy can help to unravel the diverse modes of action of AMPs. Here, we present an overview of macromolecule biosynthesis rate measurement and fluorescence spectroscopy methods to identify AMP mode(s) of action. Detailed protocols designed to measure inhibition of DNA, RNA, protein, and cell wall synthesis or membrane de-energization are presented and discussed for optimal application of these two techniques as well as to enable accurate interpretation of the experimental findings.

AB - Antimicrobial peptides (AMPs) are viable alternatives to the currently available antimicrobials, and numerous studies have investigated their possible use as therapeutic agents for specific clinical applications. AMPs are a diverse class of antimicrobials that often act upon the bacterial cell membrane but may exhibit additional modes of action. Identification of the multiple modes of action requires a comprehensive study at subinhibitory concentrations and careful data analysis since additional modes of action can be eclipsed by AMP action on the cell membrane. Techniques that measure the biosynthesis rate of macromolecules (e.g., DNA, RNA, protein, and cell wall) and the cytoplasmic membrane proton motive force (PMF) energy can help to unravel the diverse modes of action of AMPs. Here, we present an overview of macromolecule biosynthesis rate measurement and fluorescence spectroscopy methods to identify AMP mode(s) of action. Detailed protocols designed to measure inhibition of DNA, RNA, protein, and cell wall synthesis or membrane de-energization are presented and discussed for optimal application of these two techniques as well as to enable accurate interpretation of the experimental findings.

KW - Antimicrobial peptide

KW - Fluorescence spectroscopy

KW - Macromolecule biosynthesis

KW - Membrane de-energization/depolarization

KW - Mode(s) of action

KW - Proton motive force

U2 - 10.1007/978-1-4939-6737-7_12

DO - 10.1007/978-1-4939-6737-7_12

M3 - Book chapter

SN - 978-1-4939-6735-3

VL - 1548

SP - 181

EP - 190

BT - Antimicrobial Peptides

PB - Humana Press

ER -

ID: 184357303