Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes. / Albers, Peter Hjorth; Pedersen, Andreas J T; Birk, Jesper Bratz; Kristensen, Dorte Enggaard; Vind, Birgitte F; Baba, Otto; Nøhr, Jane; Højlund, Kurt; Wojtaszewski, Jørgen.

I: Diabetes, Bind 64, Nr. 2, 2015, s. 485-497.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Albers, PH, Pedersen, AJT, Birk, JB, Kristensen, DE, Vind, BF, Baba, O, Nøhr, J, Højlund, K & Wojtaszewski, J 2015, 'Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes', Diabetes, bind 64, nr. 2, s. 485-497. https://doi.org/10.2337/db14-0590

APA

Albers, P. H., Pedersen, A. J. T., Birk, J. B., Kristensen, D. E., Vind, B. F., Baba, O., ... Wojtaszewski, J. (2015). Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes. Diabetes, 64(2), 485-497. https://doi.org/10.2337/db14-0590

Vancouver

Albers PH, Pedersen AJT, Birk JB, Kristensen DE, Vind BF, Baba O o.a. Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes. Diabetes. 2015;64(2):485-497. https://doi.org/10.2337/db14-0590

Author

Albers, Peter Hjorth ; Pedersen, Andreas J T ; Birk, Jesper Bratz ; Kristensen, Dorte Enggaard ; Vind, Birgitte F ; Baba, Otto ; Nøhr, Jane ; Højlund, Kurt ; Wojtaszewski, Jørgen. / Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes. I: Diabetes. 2015 ; Bind 64, Nr. 2. s. 485-497.

Bibtex

@article{0c501dc0080f454bb686185fb0671726,
title = "Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes",
abstract = "Skeletal muscle is a heterogeneous tissue composed of different fiber types. Studies suggest that insulin-mediated glucose metabolism is different between muscle fiber types. We hypothesized that differences are due to fiber-type specific expression/regulation of insulin signaling elements and/or metabolic enzymes. Pools of type I and II fibers were prepared from biopsies of the vastus lateralis muscles from lean, obese and type 2 diabetic subjects before and after a hyperinsulinemic-euglycemic clamp. Type I fibers compared to type II fibers have higher protein levels of the insulin receptor, GLUT4, hexokinase II, glycogen synthase (GS), pyruvate dehydrogenase (PDH-E1α) and a lower protein content of Akt2, TBC1D4 and TBC1D1. In type I fibers compared to type II fibers, the phosphorylation-response to insulin was similar (TBC1D4, TBC1D1 and GS) or decreased (Akt and PDH-E1α). Phosphorylation-responses to insulin adjusted for protein level were not different between fiber types. Independently of fiber type, insulin signaling was similar (TBC1D1, GS and PDH-E1α) or decreased (Akt and TBC1D4) in muscle from patients with type 2 diabetes compared to lean and obese subjects. We conclude that human type I muscle fibers compared to type II fibers have a higher glucose handling capacity but a similar sensitivity for phosphor-regulation by insulin.",
author = "Albers, {Peter Hjorth} and Pedersen, {Andreas J T} and Birk, {Jesper Bratz} and Kristensen, {Dorte Enggaard} and Vind, {Birgitte F} and Otto Baba and Jane N{\o}hr and Kurt H{\o}jlund and J{\o}rgen Wojtaszewski",
note = "CURIS 2015 NEXS 051",
year = "2015",
doi = "10.2337/db14-0590",
language = "English",
volume = "64",
pages = "485--497",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association",
number = "2",

}

RIS

TY - JOUR

T1 - Human muscle fiber type-specific insulin signaling: Impact of obesity and type 2 diabetes

AU - Albers, Peter Hjorth

AU - Pedersen, Andreas J T

AU - Birk, Jesper Bratz

AU - Kristensen, Dorte Enggaard

AU - Vind, Birgitte F

AU - Baba, Otto

AU - Nøhr, Jane

AU - Højlund, Kurt

AU - Wojtaszewski, Jørgen

N1 - CURIS 2015 NEXS 051

PY - 2015

Y1 - 2015

N2 - Skeletal muscle is a heterogeneous tissue composed of different fiber types. Studies suggest that insulin-mediated glucose metabolism is different between muscle fiber types. We hypothesized that differences are due to fiber-type specific expression/regulation of insulin signaling elements and/or metabolic enzymes. Pools of type I and II fibers were prepared from biopsies of the vastus lateralis muscles from lean, obese and type 2 diabetic subjects before and after a hyperinsulinemic-euglycemic clamp. Type I fibers compared to type II fibers have higher protein levels of the insulin receptor, GLUT4, hexokinase II, glycogen synthase (GS), pyruvate dehydrogenase (PDH-E1α) and a lower protein content of Akt2, TBC1D4 and TBC1D1. In type I fibers compared to type II fibers, the phosphorylation-response to insulin was similar (TBC1D4, TBC1D1 and GS) or decreased (Akt and PDH-E1α). Phosphorylation-responses to insulin adjusted for protein level were not different between fiber types. Independently of fiber type, insulin signaling was similar (TBC1D1, GS and PDH-E1α) or decreased (Akt and TBC1D4) in muscle from patients with type 2 diabetes compared to lean and obese subjects. We conclude that human type I muscle fibers compared to type II fibers have a higher glucose handling capacity but a similar sensitivity for phosphor-regulation by insulin.

AB - Skeletal muscle is a heterogeneous tissue composed of different fiber types. Studies suggest that insulin-mediated glucose metabolism is different between muscle fiber types. We hypothesized that differences are due to fiber-type specific expression/regulation of insulin signaling elements and/or metabolic enzymes. Pools of type I and II fibers were prepared from biopsies of the vastus lateralis muscles from lean, obese and type 2 diabetic subjects before and after a hyperinsulinemic-euglycemic clamp. Type I fibers compared to type II fibers have higher protein levels of the insulin receptor, GLUT4, hexokinase II, glycogen synthase (GS), pyruvate dehydrogenase (PDH-E1α) and a lower protein content of Akt2, TBC1D4 and TBC1D1. In type I fibers compared to type II fibers, the phosphorylation-response to insulin was similar (TBC1D4, TBC1D1 and GS) or decreased (Akt and PDH-E1α). Phosphorylation-responses to insulin adjusted for protein level were not different between fiber types. Independently of fiber type, insulin signaling was similar (TBC1D1, GS and PDH-E1α) or decreased (Akt and TBC1D4) in muscle from patients with type 2 diabetes compared to lean and obese subjects. We conclude that human type I muscle fibers compared to type II fibers have a higher glucose handling capacity but a similar sensitivity for phosphor-regulation by insulin.

U2 - 10.2337/db14-0590

DO - 10.2337/db14-0590

M3 - Journal article

VL - 64

SP - 485

EP - 497

JO - Diabetes

T2 - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 2

ER -

ID: 123269383