High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Sarah Louise Fordyce, Maria del Carmen Avila Arcos, Eszter Rockenbauer, Claus Børsting, Rune Frank-Hansen, Johan Frederik Torp Petersen, Eske Willerslev, Anders Johannes Hansen, Niels Morling, Tom Gilbert

The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR repeat units. These methods do not allow for the full resolution of STR base composition that sequencing approaches could provide. Here we present an STR profiling method based on the use of the Roche Genome Sequencer (GS) FLX to simultaneously sequence multiple core STR loci. Using this method in combination with a bioinformatic tool designed specifically to analyze sequence lengths and frequencies, we found that GS FLX STR sequence data are comparable to conventional capillary electrophoresis-based STR typing. Furthermore, we found DNA base substitutions and repeat sequence variations that would not have been identified using conventional STR typing.
OriginalsprogEngelsk
TidsskriftBioTechniques
Vol/bind51
Udgave nummer2
Sider (fra-til)127-133
Antal sider7
ISSN0736-6205
DOI
StatusUdgivet - 1 aug. 2011

ID: 33882847