Generation of two isogenic iPSC lines with either a heterozygous or a homozygous E280A mutation in the PSEN1 gene.

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Henriette Reventlow S Frederiksen, Bjørn Holst, Ulrike A. Mau-Holzmann, Kristine Freude, Benjamin Schmid

Alzheimer's disease (AD) is the most common form of dementia. Mutations in the gene PSEN1 encoding Presenilin1 are known to cause familial forms of AD with early age of onset. The most common mutation in the PSEN1 gene is the E280A mutation. iPSCs are an optimal choice for modeling AD, as they can be differentiated in vitro into neural cells. Here, we report the generation of two isogenic iPSC lines with either a homozygous or a heterozygous E280A mutation in the PSEN1 gene. The mutation was introduced into an iPSC line from a healthy individual using the CRISPR-Cas9 technology.
OriginalsprogEngelsk
Artikelnummer101403
TidsskriftStem Cell Research
Vol/bind35
Antal sider4
ISSN1873-5061
DOI
StatusUdgivet - mar. 2019

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