Expression, content, and localization of insulin-like growth factor I in human achilles tendon

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Jens L Olesen, Katja M Heinemeier, Henning Langberg, S Peter Magnusson, Michael Kjaer, Allan Flyvbjerg

In animals insulin-like growth factor I (IGF-I) stimulates collagen production by fibroblasts and is expressed in tendons together with its binding protein 4 (IGFBP-4). However, the presence of IGF-I and IGFBP-4 in human tendon tissue is not described. Tissue IGF-I content was examined by immunoflourometric assay, real-time PCR, and immunohistochemistry used to localize and determine expression of IGF-I and IGFBP-4 in 6 postmortem human Achilles tendons. Tendon tissue concentrations of IGF-I were found to be 0.53 +/- 0.10 ng/g. Furthermore, we demonstrated that IGF-I and IGFBP-4 are localized around the tendon fibroblasts and that mRNA for IGF-I and IGFBP-4 can be determined in human tendon tissue. The present study adds support for the roles of IGF-I and IGFBP-4 in the regulation of tendon adaptive responses to mechanical loading.
OriginalsprogEngelsk
TidsskriftConnective Tissue Research
Vol/bind47
Udgave nummer4
Sider (fra-til)200-6
Antal sider7
ISSN0300-8207
DOI
StatusUdgivet - 2006

ID: 38366164