Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli

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Standard

Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli. / Mirza, Nadia Muhammad Akram; Crocoll, Christoph; Olsen, Carl Erik; Halkier, Barbara Ann.

I: Metabolic Engineering, Bind 35, 2016, s. 31-37.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Mirza, NMA, Crocoll, C, Olsen, CE & Halkier, BA 2016, 'Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli' Metabolic Engineering, bind 35, s. 31-37. https://doi.org/10.1016/j.ymben.2015.09.012

APA

Mirza, N. M. A., Crocoll, C., Olsen, C. E., & Halkier, B. A. (2016). Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli. Metabolic Engineering, 35, 31-37. https://doi.org/10.1016/j.ymben.2015.09.012

Vancouver

Mirza NMA, Crocoll C, Olsen CE, Halkier BA. Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli. Metabolic Engineering. 2016;35:31-37. https://doi.org/10.1016/j.ymben.2015.09.012

Author

Mirza, Nadia Muhammad Akram ; Crocoll, Christoph ; Olsen, Carl Erik ; Halkier, Barbara Ann. / Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli. I: Metabolic Engineering. 2016 ; Bind 35. s. 31-37.

Bibtex

@article{0f2f8e453cfd475694378f14758be5c6,
title = "Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli",
abstract = "The methionine-derived glucosinolate glucoraphanin is associated with the health-promoting properties of broccoli. This has developed a strong interest in producing this compound in high amounts from a microbial source. Glucoraphanin synthesis starts with a five-gene chain elongation pathway that converts methionine to dihomo-methionine, which is subsequently converted to glucoraphanin by the seven-gene glucosinolate core structure pathway. As dihomo-methionine is the precursor amino acid for glucoraphanin production, a first challenge is to establish an expression system for production of dihomo-methionine. In planta, the methionine chain elongation enzymes are physically separated within the cell with the first enzyme in the cytosol while the rest are located in the chloroplast. A de-compartmentalization approach was applied to produce dihomo-methionine by expression of the respective plant genes in Escherichia coli cytosol. Introduction of two plasmids encoding the methionine chain elongation pathway into E. coli resulted in production of 25mgL(-1) of dihomo-methionine. In addition to chain-elongated methionine products, side-products from chain elongation of leucine were produced. Methionine supplementation enhanced dihomo-methionine production to 57mgL(-1), while keeping a steady level of the chain-elongated leucine products. Engineering of the de-compartmentalized pathway of dihomo-methionine in E. coli cytosol provides an important first step for microbial production of the health-promoting glucoraphanin.",
author = "Mirza, {Nadia Muhammad Akram} and Christoph Crocoll and Olsen, {Carl Erik} and Halkier, {Barbara Ann}",
note = "Copyright {\circledC} 2015. Published by Elsevier Inc.",
year = "2016",
doi = "10.1016/j.ymben.2015.09.012",
language = "English",
volume = "35",
pages = "31--37",
journal = "Metabolic Engineering",
issn = "1096-7176",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Engineering of methionine chain elongation part of glucoraphanin pathway in E. coli

AU - Mirza, Nadia Muhammad Akram

AU - Crocoll, Christoph

AU - Olsen, Carl Erik

AU - Halkier, Barbara Ann

N1 - Copyright © 2015. Published by Elsevier Inc.

PY - 2016

Y1 - 2016

N2 - The methionine-derived glucosinolate glucoraphanin is associated with the health-promoting properties of broccoli. This has developed a strong interest in producing this compound in high amounts from a microbial source. Glucoraphanin synthesis starts with a five-gene chain elongation pathway that converts methionine to dihomo-methionine, which is subsequently converted to glucoraphanin by the seven-gene glucosinolate core structure pathway. As dihomo-methionine is the precursor amino acid for glucoraphanin production, a first challenge is to establish an expression system for production of dihomo-methionine. In planta, the methionine chain elongation enzymes are physically separated within the cell with the first enzyme in the cytosol while the rest are located in the chloroplast. A de-compartmentalization approach was applied to produce dihomo-methionine by expression of the respective plant genes in Escherichia coli cytosol. Introduction of two plasmids encoding the methionine chain elongation pathway into E. coli resulted in production of 25mgL(-1) of dihomo-methionine. In addition to chain-elongated methionine products, side-products from chain elongation of leucine were produced. Methionine supplementation enhanced dihomo-methionine production to 57mgL(-1), while keeping a steady level of the chain-elongated leucine products. Engineering of the de-compartmentalized pathway of dihomo-methionine in E. coli cytosol provides an important first step for microbial production of the health-promoting glucoraphanin.

AB - The methionine-derived glucosinolate glucoraphanin is associated with the health-promoting properties of broccoli. This has developed a strong interest in producing this compound in high amounts from a microbial source. Glucoraphanin synthesis starts with a five-gene chain elongation pathway that converts methionine to dihomo-methionine, which is subsequently converted to glucoraphanin by the seven-gene glucosinolate core structure pathway. As dihomo-methionine is the precursor amino acid for glucoraphanin production, a first challenge is to establish an expression system for production of dihomo-methionine. In planta, the methionine chain elongation enzymes are physically separated within the cell with the first enzyme in the cytosol while the rest are located in the chloroplast. A de-compartmentalization approach was applied to produce dihomo-methionine by expression of the respective plant genes in Escherichia coli cytosol. Introduction of two plasmids encoding the methionine chain elongation pathway into E. coli resulted in production of 25mgL(-1) of dihomo-methionine. In addition to chain-elongated methionine products, side-products from chain elongation of leucine were produced. Methionine supplementation enhanced dihomo-methionine production to 57mgL(-1), while keeping a steady level of the chain-elongated leucine products. Engineering of the de-compartmentalized pathway of dihomo-methionine in E. coli cytosol provides an important first step for microbial production of the health-promoting glucoraphanin.

U2 - 10.1016/j.ymben.2015.09.012

DO - 10.1016/j.ymben.2015.09.012

M3 - Journal article

VL - 35

SP - 31

EP - 37

JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

ER -

ID: 153047209