Deep sequencing of RNA from ancient maize kernels

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Deep sequencing of RNA from ancient maize kernels. / Fordyce, Sarah Louise; Avila Arcos, Maria del Carmen; Rasmussen, Morten; Cappellini, Enrico; Romero-Navarro, J. Alberto; Wales, Nathan; Alquezar Planas, David Eugenio; Penfield, Steven; Brown, Terence A.; Vielle-Calzada, Jean-Philippe; Montiel, Rafael; Jørgensen, Tina; Odegaard, Nancy; Jacobs, Michael; Arriaza, Bernardo; Higham, Thomas F. G.; Ramsey, Christopher Bronk; Willerslev, Eske; Gilbert, Tom.

I: PLoS ONE, Bind 8, Nr. 1, e50961, 11.01.2013.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Fordyce, SL, Avila Arcos, MDC, Rasmussen, M, Cappellini, E, Romero-Navarro, JA, Wales, N, Alquezar Planas, DE, Penfield, S, Brown, TA, Vielle-Calzada, J-P, Montiel, R, Jørgensen, T, Odegaard, N, Jacobs, M, Arriaza, B, Higham, TFG, Ramsey, CB, Willerslev, E & Gilbert, T 2013, 'Deep sequencing of RNA from ancient maize kernels' PLoS ONE, bind 8, nr. 1, e50961. https://doi.org/10.1371/journal.pone.0050961

APA

Fordyce, S. L., Avila Arcos, M. D. C., Rasmussen, M., Cappellini, E., Romero-Navarro, J. A., Wales, N., ... Gilbert, T. (2013). Deep sequencing of RNA from ancient maize kernels. PLoS ONE, 8(1), [e50961]. https://doi.org/10.1371/journal.pone.0050961

Vancouver

Fordyce SL, Avila Arcos MDC, Rasmussen M, Cappellini E, Romero-Navarro JA, Wales N o.a. Deep sequencing of RNA from ancient maize kernels. PLoS ONE. 2013 jan 11;8(1). e50961. https://doi.org/10.1371/journal.pone.0050961

Author

Fordyce, Sarah Louise ; Avila Arcos, Maria del Carmen ; Rasmussen, Morten ; Cappellini, Enrico ; Romero-Navarro, J. Alberto ; Wales, Nathan ; Alquezar Planas, David Eugenio ; Penfield, Steven ; Brown, Terence A. ; Vielle-Calzada, Jean-Philippe ; Montiel, Rafael ; Jørgensen, Tina ; Odegaard, Nancy ; Jacobs, Michael ; Arriaza, Bernardo ; Higham, Thomas F. G. ; Ramsey, Christopher Bronk ; Willerslev, Eske ; Gilbert, Tom. / Deep sequencing of RNA from ancient maize kernels. I: PLoS ONE. 2013 ; Bind 8, Nr. 1.

Bibtex

@article{975922f6d93f4e8ca8a9ab7a28fdfefb,
title = "Deep sequencing of RNA from ancient maize kernels",
abstract = "The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.",
author = "Fordyce, {Sarah Louise} and {Avila Arcos}, {Maria del Carmen} and Morten Rasmussen and Enrico Cappellini and Romero-Navarro, {J. Alberto} and Nathan Wales and {Alquezar Planas}, {David Eugenio} and Steven Penfield and Brown, {Terence A.} and Jean-Philippe Vielle-Calzada and Rafael Montiel and Tina J{\o}rgensen and Nancy Odegaard and Michael Jacobs and Bernardo Arriaza and Higham, {Thomas F. G.} and Ramsey, {Christopher Bronk} and Eske Willerslev and Tom Gilbert",
year = "2013",
month = "1",
day = "11",
doi = "10.1371/journal.pone.0050961",
language = "English",
volume = "8",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

RIS

TY - JOUR

T1 - Deep sequencing of RNA from ancient maize kernels

AU - Fordyce, Sarah Louise

AU - Avila Arcos, Maria del Carmen

AU - Rasmussen, Morten

AU - Cappellini, Enrico

AU - Romero-Navarro, J. Alberto

AU - Wales, Nathan

AU - Alquezar Planas, David Eugenio

AU - Penfield, Steven

AU - Brown, Terence A.

AU - Vielle-Calzada, Jean-Philippe

AU - Montiel, Rafael

AU - Jørgensen, Tina

AU - Odegaard, Nancy

AU - Jacobs, Michael

AU - Arriaza, Bernardo

AU - Higham, Thomas F. G.

AU - Ramsey, Christopher Bronk

AU - Willerslev, Eske

AU - Gilbert, Tom

PY - 2013/1/11

Y1 - 2013/1/11

N2 - The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.

AB - The characterization of biomolecules from ancient samples can shed otherwise unobtainable insights into the past. Despite the fundamental role of transcriptomal change in evolution, the potential of ancient RNA remains unexploited - perhaps due to dogma associated with the fragility of RNA. We hypothesize that seeds offer a plausible refuge for long-term RNA survival, due to the fundamental role of RNA during seed germination. Using RNA-Seq on cDNA synthesized from nucleic acid extracts, we validate this hypothesis through demonstration of partial transcriptomal recovery from two sources of ancient maize kernels. The results suggest that ancient seed transcriptomics may offer a powerful new tool with which to study plant domestication.

U2 - 10.1371/journal.pone.0050961

DO - 10.1371/journal.pone.0050961

M3 - Journal article

VL - 8

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 1

M1 - e50961

ER -

ID: 46089758