Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent

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Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent. / Jeppesen, Jacob; Albers, Peter Hjorth; Rose, Adam John; Birk, Jesper Bratz; Schjerling, Peter; Dzamko, Nicolas; Steinberg, Gregory R.; Kiens, Bente.

I: Journal of Lipid Research, Bind 52, Nr. 4, 2011, s. 699-711.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Jeppesen, J, Albers, PH, Rose, AJ, Birk, JB, Schjerling, P, Dzamko, N, Steinberg, GR & Kiens, B 2011, 'Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent', Journal of Lipid Research, bind 52, nr. 4, s. 699-711. https://doi.org/10.1194/jlr.M007138

APA

Jeppesen, J., Albers, P. H., Rose, A. J., Birk, J. B., Schjerling, P., Dzamko, N., ... Kiens, B. (2011). Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent. Journal of Lipid Research, 52(4), 699-711. https://doi.org/10.1194/jlr.M007138

Vancouver

Jeppesen J, Albers PH, Rose AJ, Birk JB, Schjerling P, Dzamko N o.a. Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent. Journal of Lipid Research. 2011;52(4):699-711. https://doi.org/10.1194/jlr.M007138

Author

Jeppesen, Jacob ; Albers, Peter Hjorth ; Rose, Adam John ; Birk, Jesper Bratz ; Schjerling, Peter ; Dzamko, Nicolas ; Steinberg, Gregory R. ; Kiens, Bente. / Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent. I: Journal of Lipid Research. 2011 ; Bind 52, Nr. 4. s. 699-711.

Bibtex

@article{c60f35418c3840aab465f2f4971c5060,
title = "Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent",
abstract = "The aim of this study was to investigate the molecular mechanisms regulating FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. In one model, WT and AMPK KD mice were exercised or EDL and SOL muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and fatty acid uptake in response to muscle contractions was investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34{\%}) and AMPK KD (+37{\%}) mice. In contrast, AICAR only induced an increase in cell surface FAT/CD36 content in WT (+29{\%}) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15{\%}) and sustained (10 min, +24{\%}) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions associated with increased fatty acid uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.",
author = "Jacob Jeppesen and Albers, {Peter Hjorth} and Rose, {Adam John} and Birk, {Jesper Bratz} and Peter Schjerling and Nicolas Dzamko and Steinberg, {Gregory R.} and Bente Kiens",
note = "CURIS 2011 5200 026",
year = "2011",
doi = "10.1194/jlr.M007138",
language = "English",
volume = "52",
pages = "699--711",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Contraction-induced skeletal muscle FAT/CD36 trafficking and FA uptake is AMPK independent

AU - Jeppesen, Jacob

AU - Albers, Peter Hjorth

AU - Rose, Adam John

AU - Birk, Jesper Bratz

AU - Schjerling, Peter

AU - Dzamko, Nicolas

AU - Steinberg, Gregory R.

AU - Kiens, Bente

N1 - CURIS 2011 5200 026

PY - 2011

Y1 - 2011

N2 - The aim of this study was to investigate the molecular mechanisms regulating FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. In one model, WT and AMPK KD mice were exercised or EDL and SOL muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and fatty acid uptake in response to muscle contractions was investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, AICAR only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions associated with increased fatty acid uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.

AB - The aim of this study was to investigate the molecular mechanisms regulating FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. In one model, WT and AMPK KD mice were exercised or EDL and SOL muscles were contracted, ex vivo. In separate studies, FAT/CD36 translocation and fatty acid uptake in response to muscle contractions was investigated in the perfused rat hindlimb. Exercise induced a similar increase in skeletal muscle cell surface membrane FAT/CD36 content in WT (+34%) and AMPK KD (+37%) mice. In contrast, AICAR only induced an increase in cell surface FAT/CD36 content in WT (+29%) mice. Furthermore, in the perfused rat hindlimb, muscle contraction induced a rapid (1 min, +15%) and sustained (10 min, +24%) FAT/CD36 relocation to cell surface membranes. The increase in cell surface FAT/CD36 protein content with muscle contractions associated with increased fatty acid uptake, both in EDL and SOL muscle from WT and AMPK KD mice and in the perfused rat hindlimb. This suggests that AMPK is not essential in regulation of FAT/CD36 translocation and fatty acid uptake in skeletal muscle during contractions. However, AMPK could be important in regulation of FAT/CD36 distribution in other physiological situations.

U2 - 10.1194/jlr.M007138

DO - 10.1194/jlr.M007138

M3 - Journal article

VL - 52

SP - 699

EP - 711

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 4

ER -

ID: 32928268