Cell-based lipid flippase assay employing fluorescent lipid derivatives

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Maria Stumph Jensen, Sara Costa, Thomas Günther-Pomorski, Rosa Laura Lopez Marques

P-type ATPases in the P4 subfamily (P4-ATPases) are transmembrane proteins unique for eukaryotes that act as lipid flippases, i.e., to translocate phospholipids from the exofacial to the cytofacial monolayer of cellular membranes. While initially characterized as aminophospholipid translocases, studies of individual P4-ATPase family members from fungi, plants, and animals show that P4-ATPases differ in their substrate specificities and mediate transport of a broader range of lipid substrates. Here, we describe an assay based on fluorescent lipid derivatives to monitor and characterize lipid flippase activities in the plasma membrane of cells, using yeast as an example.

OriginalsprogEngelsk
TitelP-Type ATPases : methods and protocols
RedaktørerMaike Bublitz
Antal sider12
Vol/bindVI
ForlagSpringer
Publikationsdato2016
Sider371-382
Kapitel33
ISBN (Trykt)978-1-4939-3178-1
ISBN (Elektronisk)978-1-4939-3179-8
DOI
StatusUdgivet - 2016
NavnMethods in Molecular Biology
Vol/bind1377
ISSN1064-3745

ID: 169355616