Find en forsker – Københavns Universitet

A strand specific high resolution normalization method for chip-sequencing data employing multiple experimental control measurements.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Dokumenter

  • A strand...

    Forlagets udgivne version, 556 KB, PDF-dokument

Stefan Enroth, Claes Andersson, Robin Andersson, Claes Wadelius, Mats Gustafsson, Jan Komorowski

High-throughput sequencing is becoming the standard tool for investigating protein-DNA interactions or epigenetic modifications. However, the data generated will always contain noise due to e.g. repetitive regions or non-specific antibody interactions. The noise will appear in the form of a background distribution of reads that must be taken into account in the downstream analysis, for example when detecting enriched regions (peak-calling). Several reported peak-callers can take experimental measurements of background tag distribution into account when analysing a data set. Unfortunately, the background is only used to adjust peak calling and not as a pre-processing step that aims at discerning the signal from the background noise. A normalization procedure that extracts the signal of interest would be of universal use when investigating genomic patterns.
OriginalsprogEngelsk
TidsskriftAlgorithms for Molecular Biology
Vol/bind7
Tidsskriftsnummer1
Sider (fra-til)2
Antal sider10
ISSN1748-7188
DOI
StatusUdgivet - 2012

Antal downloads er baseret på statistik fra Google Scholar og www.ku.dk


Ingen data tilgængelig

ID: 43869460