A comparative study of ancient environmental DNA to pollen and macrofossils from lake sediments reveals taxonomic overlap and additional plant taxa

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Mikkel Winther Pedersen, Aurélien Ginolhac, Ludovic Antoine Alexandre Orlando, Jesper Olsen, Kenneth Andersen, Jakob Mørkøv Holm, Svend Visby Funder, Eske Willerslev, Kurt H. Kjær

We use 2nd generation sequencing technology on sedimentary ancient DNA (. sedaDNA) from a lake in South Greenland to reconstruct the local floristic history around a low-arctic lake and compare the results with those previously obtained from pollen and macrofossils in the same lake. Thirty-eight of thirty-nine samples from the core yielded putative DNA sequences. Using a multiple assignment strategy on the trnL g-h DNA barcode, consisting of two different phylogenetic and one sequence similarity assignment approaches, thirteen families of plants were identified, of which two (. Scrophulariaceae and Asparagaceae) are absent from the pollen and macrofossil records. An age model for the sediment based on twelve radiocarbon dates establishes a chronology and shows that the lake record dates back to 10,650calyrBP. Our results suggest that sedaDNA analysis from lake sediments, although taxonomically less detailed than pollen and macrofossil analyses can be a complementary tool for establishing the composition of both terrestrial and aquatic local plant communities and a method for identifying additional taxa.
OriginalsprogEngelsk
TidsskriftQuaternary Science Reviews
Vol/bind75
Sider (fra-til)161-168
Antal sider8
ISSN0277-3791
DOI
StatusUdgivet - 1 sep. 2013

ID: 91795497