Standard
Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas. / Bender, Sebastian; Tang, Yujie; Lindroth, Anders M; Hovestadt, Volker; Jones, David T W; Kool, Marcel; Zapatka, Marc; Northcott, Paul A; Sturm, Dominik; Wang, Wei Guo; Radlwimmer, Bernhard; Højfeldt, Jonas W; Truffaux, Nathalène; Castel, David; Schubert, Simone; Ryzhova, Marina; Seker-Cin, Huriye; Gronych, Jan; Johann, Pascal David; Stark, Sebastian; Meyer, Jochen; Milde, Till; Schuhmann, Martin; Ebinger, Martin; Monoranu, Camelia-Maria; Ponnuswami, Anitha; Chen, Spenser; Jones, Chris; Witt, Olaf; Collins, V Peter; von Deimling, Andreas; Jabado, Nada; Puget, Stephanie; Grill, Jacques; Helin, Kristian; Korshunov, Andrey; Lichter, Peter; Monje, Michelle; Plass, Christoph; Cho, Yoon-Jae; Pfister, Stefan M.
In:
Cancer Cell, Vol. 24, No. 5, 11.11.2013, p. 660-72.
Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
Bender, S, Tang, Y, Lindroth, AM, Hovestadt, V, Jones, DTW, Kool, M, Zapatka, M, Northcott, PA, Sturm, D, Wang, WG, Radlwimmer, B, Højfeldt, JW, Truffaux, N, Castel, D, Schubert, S, Ryzhova, M, Seker-Cin, H, Gronych, J, Johann, PD, Stark, S, Meyer, J, Milde, T, Schuhmann, M, Ebinger, M, Monoranu, C-M, Ponnuswami, A, Chen, S, Jones, C, Witt, O, Collins, VP, von Deimling, A, Jabado, N, Puget, S, Grill, J, Helin, K, Korshunov, A, Lichter, P, Monje, M, Plass, C, Cho, Y-J & Pfister, SM 2013, '
Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas',
Cancer Cell, vol. 24, no. 5, pp. 660-72.
https://doi.org/10.1016/j.ccr.2013.10.006APA
Bender, S., Tang, Y., Lindroth, A. M., Hovestadt, V., Jones, D. T. W., Kool, M., Zapatka, M., Northcott, P. A., Sturm, D., Wang, W. G., Radlwimmer, B., Højfeldt, J. W., Truffaux, N., Castel, D., Schubert, S., Ryzhova, M., Seker-Cin, H., Gronych, J., Johann, P. D., ... Pfister, S. M. (2013).
Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas.
Cancer Cell,
24(5), 660-72.
https://doi.org/10.1016/j.ccr.2013.10.006Vancouver
Bender S, Tang Y, Lindroth AM, Hovestadt V, Jones DTW, Kool M et al.
Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas.
Cancer Cell. 2013 Nov 11;24(5):660-72.
https://doi.org/10.1016/j.ccr.2013.10.006Author
Bender, Sebastian ; Tang, Yujie ; Lindroth, Anders M ; Hovestadt, Volker ; Jones, David T W ; Kool, Marcel ; Zapatka, Marc ; Northcott, Paul A ; Sturm, Dominik ; Wang, Wei Guo ; Radlwimmer, Bernhard ; Højfeldt, Jonas W ; Truffaux, Nathalène ; Castel, David ; Schubert, Simone ; Ryzhova, Marina ; Seker-Cin, Huriye ; Gronych, Jan ; Johann, Pascal David ; Stark, Sebastian ; Meyer, Jochen ; Milde, Till ; Schuhmann, Martin ; Ebinger, Martin ; Monoranu, Camelia-Maria ; Ponnuswami, Anitha ; Chen, Spenser ; Jones, Chris ; Witt, Olaf ; Collins, V Peter ; von Deimling, Andreas ; Jabado, Nada ; Puget, Stephanie ; Grill, Jacques ; Helin, Kristian ; Korshunov, Andrey ; Lichter, Peter ; Monje, Michelle ; Plass, Christoph ; Cho, Yoon-Jae ; Pfister, Stefan M. / Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas. In: Cancer Cell. 2013 ; Vol. 24, No. 5. pp. 660-72.
Bibtex
@article{b3f5cab317d14c5899d4e8678dbea1df,
title = "Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas",
abstract = "Two recurrent mutations, K27M and G34R/V, within histone variant H3.3 were recently identified in ∼50% of pHGGs. Both mutations define clinically and biologically distinct subgroups of pHGGs. Here, we provide further insight about the dominant-negative effect of K27M mutant H3.3, leading to a global reduction of the repressive histone mark H3K27me3. We demonstrate that this is caused by aberrant recruitment of the PRC2 complex to K27M mutant H3.3 and enzymatic inhibition of the H3K27me3-establishing methyltransferase EZH2. By performing chromatin immunoprecipitation followed by next-generation sequencing and whole-genome bisulfite sequencing in primary pHGGs, we show that reduced H3K27me3 levels and DNA hypomethylation act in concert to activate gene expression in K27M mutant pHGGs.",
author = "Sebastian Bender and Yujie Tang and Lindroth, {Anders M} and Volker Hovestadt and Jones, {David T W} and Marcel Kool and Marc Zapatka and Northcott, {Paul A} and Dominik Sturm and Wang, {Wei Guo} and Bernhard Radlwimmer and H{\o}jfeldt, {Jonas W} and Nathal{\`e}ne Truffaux and David Castel and Simone Schubert and Marina Ryzhova and Huriye Seker-Cin and Jan Gronych and Johann, {Pascal David} and Sebastian Stark and Jochen Meyer and Till Milde and Martin Schuhmann and Martin Ebinger and Camelia-Maria Monoranu and Anitha Ponnuswami and Spenser Chen and Chris Jones and Olaf Witt and Collins, {V Peter} and {von Deimling}, Andreas and Nada Jabado and Stephanie Puget and Jacques Grill and Kristian Helin and Andrey Korshunov and Peter Lichter and Michelle Monje and Christoph Plass and Yoon-Jae Cho and Pfister, {Stefan M}",
note = "Copyright {\textcopyright} 2013 Elsevier Inc. All rights reserved.",
year = "2013",
month = nov,
day = "11",
doi = "10.1016/j.ccr.2013.10.006",
language = "English",
volume = "24",
pages = "660--72",
journal = "Cancer Cell",
issn = "1535-6108",
publisher = "Cell Press",
number = "5",
}
RIS
TY - JOUR
T1 - Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas
AU - Bender, Sebastian
AU - Tang, Yujie
AU - Lindroth, Anders M
AU - Hovestadt, Volker
AU - Jones, David T W
AU - Kool, Marcel
AU - Zapatka, Marc
AU - Northcott, Paul A
AU - Sturm, Dominik
AU - Wang, Wei Guo
AU - Radlwimmer, Bernhard
AU - Højfeldt, Jonas W
AU - Truffaux, Nathalène
AU - Castel, David
AU - Schubert, Simone
AU - Ryzhova, Marina
AU - Seker-Cin, Huriye
AU - Gronych, Jan
AU - Johann, Pascal David
AU - Stark, Sebastian
AU - Meyer, Jochen
AU - Milde, Till
AU - Schuhmann, Martin
AU - Ebinger, Martin
AU - Monoranu, Camelia-Maria
AU - Ponnuswami, Anitha
AU - Chen, Spenser
AU - Jones, Chris
AU - Witt, Olaf
AU - Collins, V Peter
AU - von Deimling, Andreas
AU - Jabado, Nada
AU - Puget, Stephanie
AU - Grill, Jacques
AU - Helin, Kristian
AU - Korshunov, Andrey
AU - Lichter, Peter
AU - Monje, Michelle
AU - Plass, Christoph
AU - Cho, Yoon-Jae
AU - Pfister, Stefan M
N1 - Copyright © 2013 Elsevier Inc. All rights reserved.
PY - 2013/11/11
Y1 - 2013/11/11
N2 - Two recurrent mutations, K27M and G34R/V, within histone variant H3.3 were recently identified in ∼50% of pHGGs. Both mutations define clinically and biologically distinct subgroups of pHGGs. Here, we provide further insight about the dominant-negative effect of K27M mutant H3.3, leading to a global reduction of the repressive histone mark H3K27me3. We demonstrate that this is caused by aberrant recruitment of the PRC2 complex to K27M mutant H3.3 and enzymatic inhibition of the H3K27me3-establishing methyltransferase EZH2. By performing chromatin immunoprecipitation followed by next-generation sequencing and whole-genome bisulfite sequencing in primary pHGGs, we show that reduced H3K27me3 levels and DNA hypomethylation act in concert to activate gene expression in K27M mutant pHGGs.
AB - Two recurrent mutations, K27M and G34R/V, within histone variant H3.3 were recently identified in ∼50% of pHGGs. Both mutations define clinically and biologically distinct subgroups of pHGGs. Here, we provide further insight about the dominant-negative effect of K27M mutant H3.3, leading to a global reduction of the repressive histone mark H3K27me3. We demonstrate that this is caused by aberrant recruitment of the PRC2 complex to K27M mutant H3.3 and enzymatic inhibition of the H3K27me3-establishing methyltransferase EZH2. By performing chromatin immunoprecipitation followed by next-generation sequencing and whole-genome bisulfite sequencing in primary pHGGs, we show that reduced H3K27me3 levels and DNA hypomethylation act in concert to activate gene expression in K27M mutant pHGGs.
U2 - 10.1016/j.ccr.2013.10.006
DO - 10.1016/j.ccr.2013.10.006
M3 - Journal article
C2 - 24183680
VL - 24
SP - 660
EP - 672
JO - Cancer Cell
JF - Cancer Cell
SN - 1535-6108
IS - 5
ER -
