Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum. / Zervens, Lisa Marie-Louise; Nielsen, Søren Saxmose; Jungersen, Gregers.

In: Veterinary Journal, Vol. 197, No. 3, 2013, p. 889-891.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zervens, LM-L, Nielsen, SS & Jungersen, G 2013, 'Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum', Veterinary Journal, vol. 197, no. 3, pp. 889-891. https://doi.org/10.1016/j.tvjl.2013.03.018

APA

Zervens, L. M-L., Nielsen, S. S., & Jungersen, G. (2013). Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum. Veterinary Journal, 197(3), 889-891. https://doi.org/10.1016/j.tvjl.2013.03.018

Vancouver

Zervens LM-L, Nielsen SS, Jungersen G. Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum. Veterinary Journal. 2013;197(3):889-891. https://doi.org/10.1016/j.tvjl.2013.03.018

Author

Zervens, Lisa Marie-Louise ; Nielsen, Søren Saxmose ; Jungersen, Gregers. / Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum. In: Veterinary Journal. 2013 ; Vol. 197, No. 3. pp. 889-891.

Bibtex

@article{98c2313734d34a51ba3b21d52d2cd932,

title = "Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum",

abstract = "Although colostrum has been used to detect specific immunoglobulin (Ig) G to Mycobacterium avium subsp. paratuberculosis (MAP) in cattle, confounding, non-specific reactions can be a problem. The objectives of this study were to determine the proportion of non-specific ELISA reactions in samples of colostrum taken between 0 and 4days-in-milk (DIM), and to assess the probability of an animal testing positive for MAP specific IgG over this time-period. Non-specific reactions were found in 3/365 (0.8%) of samples. The odds of an animal testing positive on day of calving were 130 times higher than at 4 DIM. The findings suggest colostral samples may have enhanced diagnostic potential over milk samples in determining if cattle have been exposed to or infected with MAP.",

author = "Zervens, {Lisa Marie-Louise} and Nielsen, {S{\o}ren Saxmose} and Gregers Jungersen",

year = "2013",

doi = "10.1016/j.tvjl.2013.03.018",

language = "English",

volume = "197",

pages = "889--891",

journal = "The Veterinary Journal",

issn = "1090-0233",

publisher = "Elsevier",

number = "3",

}

RIS

TY - JOUR

T1 - Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum

AU - Zervens, Lisa Marie-Louise

AU - Nielsen, Søren Saxmose

AU - Jungersen, Gregers

PY - 2013

Y1 - 2013

N2 - Although colostrum has been used to detect specific immunoglobulin (Ig) G to Mycobacterium avium subsp. paratuberculosis (MAP) in cattle, confounding, non-specific reactions can be a problem. The objectives of this study were to determine the proportion of non-specific ELISA reactions in samples of colostrum taken between 0 and 4days-in-milk (DIM), and to assess the probability of an animal testing positive for MAP specific IgG over this time-period. Non-specific reactions were found in 3/365 (0.8%) of samples. The odds of an animal testing positive on day of calving were 130 times higher than at 4 DIM. The findings suggest colostral samples may have enhanced diagnostic potential over milk samples in determining if cattle have been exposed to or infected with MAP.

AB - Although colostrum has been used to detect specific immunoglobulin (Ig) G to Mycobacterium avium subsp. paratuberculosis (MAP) in cattle, confounding, non-specific reactions can be a problem. The objectives of this study were to determine the proportion of non-specific ELISA reactions in samples of colostrum taken between 0 and 4days-in-milk (DIM), and to assess the probability of an animal testing positive for MAP specific IgG over this time-period. Non-specific reactions were found in 3/365 (0.8%) of samples. The odds of an animal testing positive on day of calving were 130 times higher than at 4 DIM. The findings suggest colostral samples may have enhanced diagnostic potential over milk samples in determining if cattle have been exposed to or infected with MAP.

U2 - 10.1016/j.tvjl.2013.03.018

DO - 10.1016/j.tvjl.2013.03.018

M3 - Journal article

C2 - 23611487

VL - 197

SP - 889

EP - 891

JO - The Veterinary Journal

JF - The Veterinary Journal

SN - 1090-0233

IS - 3

ER -

ID: 45434680

Forskning