Chemo-enzymatic production of O-glycopeptides for the detection of serum glycopeptide antibodies
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Protein microarray is a highly sensitive tool for antibody detection in serum. Monitoring of patients' antibody titers to specific antigens is increasingly employed in the diagnosis of several conditions, ranging from infectious diseases, allergies, autoimmune diseases, and cancer. In this protocol we present a detailed method for enzymatic generation of disease-specific O-glycopeptides and how to monitor the antibody response to these in serum using microarray technology.
Original language | English |
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Title of host publication | Immunoproteomics : Methods in Molecular Biology |
Editors | Kelly M. Fulton, Susan M. Twine |
Number of pages | 13 |
Volume | 1061 |
Publisher | Humana Press |
Publication date | 2013 |
Pages | 167-79 |
Chapter | 10 |
ISBN (Print) | 978-1-62703-589-7 |
ISBN (Electronic) | 978-1-62703-589-7 |
DOIs | |
Publication status | Published - 2013 |
Series | Methods in molecular biology (Clifton, N.J.) |
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- Animals, Antibodies, Enzymes, Glycopeptides, Humans, Protein Array Analysis
Research areas
ID: 108783865