Synthesis of O-glycopeptides and construction of glycopeptide microarrays
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
O-glycosylation of proteins is an important modification which affects biological function and immunity. In this chapter, we provide protocols for efficient solid-phase O-glycopeptide synthesis (SPGPS) and protocols for the construction of glycopeptide microarray chips for screening applications. This will be exemplified for mucin-type glycopeptides and the construction of glycopeptide microarrays. To this end, the protocols provided are particularly suited for small-scale robotic parallel synthesis. N-Terminal amine capping of deletion peptides during synthesis stands out as vital to this strategy. It allows for direct on-slide enrichment of the full-length target product and thereby bypasses tedious isolation and purification procedures.
Original language | English |
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Title of host publication | Peptide synthesis and applications |
Editors | Knud J. Jensen, Pernille Tofteng Shelton, Søren L. Pedersen |
Number of pages | 14 |
Publisher | Humana Press |
Publication date | 2013 |
Pages | 201-214 |
Chapter | 14 |
ISBN (Print) | 978-1-62703-543-9 |
DOIs | |
Publication status | Published - 2013 |
Series | Methods in Molecular Biology |
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Volume | 1047 |
ISSN | 1064-3745 |
- Amino Acids, Fluorenes, Glycopeptides, Polysaccharides, Protein Array Analysis, Solid-Phase Synthesis Techniques
Research areas
ID: 103651257