Monoclonal antibodies for the detection of Puccinia striiformis urediniospores

Research output: Contribution to journalJournal articlepeer-review

  • Peter Durand Skottrup
  • Hanne Frøkiær
  • Stephen Hearty
  • Richard O'Kennedy
  • Jørn Hejgaard
  • Mogens Nicolaisen
  • Annemarie Fejer Justesen
The fungal pathogen Pst causes yellow rust disease in wheat plants leading to crop losses. The organism spreads by releasing wind-dispersed urediniospores from infected plants. In this study a library of novel monoclonal antibodies (mAbs) was developed against Pst urediniospores. Nine mAb-producing cell lines were cloned and their cross-reactivities characterised against a panel of airborne fungal spores representing genera commonly found in the same environment as Pst. Two specific mAbs were used to develop a competitive ELISA (Pst mAb4) and a subtractive inhibition ELISA (Pst mAb8). Standard curves for both assays had good intra- and interday reproducibility. The subtractive inhibition ELISA had greater sensitivity with a detection limit of 1.5 105 spores ml1. Cross-reactivity studies of Pst mAb8 in the subtractive inhibition ELISA, showed reaction with other Puccinia spores only, suggesting that common epitopes exist within this genus. The biosensor-compatible Pst mAb8 assay principle developed in this study has the potential to be implemented in future ⿿label-free⿿ in-the-field systems for Pst detection.
Original languageEnglish
JournalMycological Research
Volume111
Pages (from-to)332-338
Number of pages7
ISSN0953-7562
Publication statusPublished - 2007

ID: 33226422