The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast
Research output: Contribution to journal › Journal article › Research › peer-review
Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus, to specific DNA sequences called Ter. Here, we demonstrate that Tus-Ter modules also induce polar RF pausing when engineered into the Saccharomyces cerevisiae genome. This heterologous RF barrier is distinct from a number of previously characterized, protein-mediated, RF pause sites in yeast, as it is neither Tof1-dependent nor counteracted by the Rrm3 helicase. Although the yeast replisome can overcome RF pausing at Tus-Ter modules, this event triggers site-specific homologous recombination that requires the RecQ helicase, Sgs1, for its timely resolution. We propose that Tus-Ter can be utilized as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications.
Original language | English |
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Article number | 3574 |
Journal | Nature Communications |
Volume | 5 |
Pages (from-to) | 1-10 |
Number of pages | 10 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 2014 |
ID: 108776990